Singular value decomposition with weighting matrix applied for optical-resolution photoacoustic microscopes.

The prestige target selectivity and imaging depth of optical-resolution photoacoustic microscope (OR-PAM) have gained attentions to enable advanced intra-cellular visualizations. However, the broad-band nature of photoacoustic signals is prone to noise and artifacts caused by the inefficient light-to-pressure translation, resulting in poor image quality. The present study foresees application of singular value decomposition (SVD) to effectively extract the photoacoustic signals from these noise and artifacts. Although spatiotemporal SVD succeeded in ultrasound flow signal extraction, the conventional multi frame model is not suitable for data acquired with scanning OR-PAM due to the burden of accessing multiple frames. To utilize SVD on the OR-PAM, this study began with exploring SVD applied on multiple A-lines of photoacoustic signal instead of frames. Upon explorations, an obstacle of uncertain presence of unwanted singular vectors was observed. To tackle this, a data-driven weighting matrix was designed to extract relevant singular vectors based on the analyses of temporal-spatial singular vectors. Evaluation on the extraction capability by the SVD with the weighting matrix showed a superior signal quality with efficient computation against past studies. In summary, this study contributes to the field by providing exploration of SVD applied on A-line signals as well as its practical utilization to distinguish and recover photoacoustic signals from noise and artifact components.