Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
Nanjing University of Chinese Medicine, Nanjing 210023, China.
Pestic Biochem Physiol. 2024 Aug;203:106007. doi: 10.1016/j.pestbp.2024.106007. Epub 2024 Jul 1.
Gray mold, caused by Botrytis cinerea is an intractable fungal disease that causes extensive damage to agricultural products. In the search for novel antifungal active ingredients, we discovered a linear pyranocoumarin Pd-D-V was effective against B. cinerea in both in vitro and in vivo assays. Furthermore, this study investigated the effects of Ca and the Ca-calcineurin signaling pathway on its antifungal activity against B. cinerea. The results indicated that Pd-D-V reduced the concentration of Ca in the mycelia of B. cinerea; CaCl, the Ca channel blocker verapamil, or the calcineurin inhibitor cyclosporin A could affect the sensitivity of Pd-D-V against B. cinerea; the expression of genes (Bccch1, Bcmid1, BccnA, Bccnb1, Bcpmc1, and Bcpmr1) of the Ca-calcineurin signaling pathway decreased after Pd-D-V treatment. In summary, Pd-D-V is compound for developing fungicides against B. cinerea. Pd-D-V can reduce intracellular Ca concentration and disturb Ca homeostasis. The Ca-calcineurin signaling pathway is important in the antifungal activity of Pd-D-V against B. cinerea.
灰霉病是由灰葡萄孢引起的一种难以治疗的真菌病，它会对农产品造成广泛的损害。在寻找新型抗真菌活性成分的过程中，我们发现一种线性吡喃香豆素 Pd-D-V 在体外和体内试验中均对灰葡萄孢有效。此外，本研究还研究了 Ca 和 Ca-钙调神经磷酸酶信号通路对其抗灰葡萄孢活性的影响。结果表明，Pd-D-V 降低了灰葡萄孢菌丝体中的 Ca 浓度；CaCl2、钙通道阻滞剂维拉帕米或钙调神经磷酸酶抑制剂环孢菌素 A 均可影响 Pd-D-V 对灰葡萄孢的敏感性；Pd-D-V 处理后，Ca-钙调神经磷酸酶信号通路的基因（Bccch1、Bcmid1、BccnA、Bccnb1、Bcpmc1 和 Bcpmr1）的表达降低。综上所述，Pd-D-V 是一种开发针对灰葡萄孢的杀菌剂的化合物。Pd-D-V 可以降低细胞内 Ca 浓度并扰乱 Ca 稳态。Ca-钙调神经磷酸酶信号通路在 Pd-D-V 对灰葡萄孢的抗真菌活性中起重要作用。
