钙/钙调蛋白信号通路在Seselin 抗真菌活性中的关键作用。
Crucial Role of the Ca/CN Signaling Pathway in the Antifungal Activity of Seselin against .
机构信息
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, Jiangsu, China.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
出版信息
J Agric Food Chem. 2023 Jun 28;71(25):9772-9781. doi: 10.1021/acs.jafc.3c01474. Epub 2023 Jun 14.
causes gray mold in many fruit and vegetable crops. We previously found that Seselin (SL) displayed antifungal activity against (EC = 6.1 μg·mL), and this study investigated the effects of Ca and the Ca/CN signaling pathway on its antifungal activity against . The results indicated that exogenous Ca, Cyclosporine A, and Verapamil reduced the sensitivity of SL against ; SL significantly reduced the intracellular Ca concentration in the hyphae; the sensitivity of strains Δ1 and Δ1 to SL were significantly increased; and the expressions of 1, 1, , 1, and 1 genes of the Ca/CN signaling pathway were significantly downregulated by SL treatment. Hence, SL is a potential compound for developing fungicides against . SL dramatically reduces intracellular Ca concentration and disturbs Ca homeostasis, leading to cell death. The Ca/CN signaling pathway plays an important role in the antifungal activity of SL against .
在许多水果和蔬菜作物中引起灰霉病。我们之前发现Seselin (SL) 对 显示出抗真菌活性（EC = 6.1 μg·mL），本研究调查了 Ca 和 Ca/CN 信号通路对其抗真菌活性的影响。结果表明，外源 Ca、环孢菌素 A 和维拉帕米降低了 SL 对 的敏感性；SL 显著降低了菌丝内的细胞内 Ca 浓度；菌株 Δ1 和 Δ1 对 SL 的敏感性显著增加；SL 处理显著下调了 Ca/CN 信号通路的 1、1、1、1 和 1 基因的表达。因此，SL 是开发防治 的杀菌剂的潜在化合物。SL 可显著降低细胞内 Ca 浓度并扰乱 Ca 稳态，导致细胞死亡。Ca/CN 信号通路在 SL 抗 中的抗真菌活性中起重要作用。
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