Synergistic effect of Cydonia oblonga and its extracted silver nanoparticles for improving antioxidant and antibacterial activity of 3D printed alginate-based hydrogel as wound dressing.

The current research studies the synergistic effect of Cydonia oblonga and its extracted nano bio‑silver as a natural and eco-friendly agent for the improvement of three-dimensional (3D)-printed alginate wound dressings. Therefore, Cydonia oblonga extract was first prepared and silver nanoparticles were extracted from it through a green and simple method. The Cydonia oblonga and its extracted bio-based nanoparticles were then added to 3D printing alginate-based ink. Subsequently, a 3D structural extrusion printer was employed to create the porous hydrogel-based wound dressings. The morphological investigation demonstrated that using the extraction method the bio-based silver nanoparticles were successfully prepared, having an average size of 17.95 ± 4.50 nm. The Cydonia oblonga extract showed comparable antioxidant activity to the commercial antioxidant and an excellent total phenol content. In addition, the results showed the combination of Cydonia oblonga extracts/silver nanoparticles significantly improved the antibacterial performance of alginate-based bioinks. In vivo, and in vitro studies confirmed their biocompatibility and significant efficacy in the treatment of burn wounds.