Matre R, Tönder O, Wesenberg F
J Immunol Methods. 1980;39(1-2):25-9. doi: 10.1016/0022-1759(80)90290-2.
Sheep erythrocytes (E), sensitized with unheated rabbit antiserum containing IgG antibodies (EA), bound human C4 when heat-inactivated serum was used as the source of complement (EAC). The EA were not agglutinated by antiserum to rabbit C3 and were not lysed in the presence of C4-deficient guinea pig serum. The EAC were agglutinated by antiserum to human C4, lysed by the addition of C4-deficient serum and were reactive in immune adherence. They were not agglutinated by antiserum to human C3. Accordingly, the cells could be designated as EAC14. They were stable and could be stored at 4 degrees C for at least 1 week without haemolysis occurring, and with no loss of reactivity in immune adherence test. E sensitized in the presence of EDTA or with heat-inactivated rabbit serum did not bind C4.
用含有IgG抗体的未加热兔抗血清致敏的绵羊红细胞（E），当使用热灭活血清作为补体来源时（EAC），能结合人C4。EA不被抗兔C3血清凝集，在缺乏C4的豚鼠血清存在时也不被溶解。EAC被抗人C4血清凝集，加入缺乏C4的血清后被溶解，并且在免疫黏附中具有反应性。它们不被抗人C3血清凝集。因此，这些细胞可被命名为EAC14。它们很稳定，可在4℃保存至少1周而不发生溶血，并且在免疫黏附试验中反应性无损失。在EDTA存在下或用热灭活兔血清致敏的E不结合C4。
