UMR 1366, Univ. Bordeaux, Bordeaux INP, INRAE, ISVV, 33140, Villenave d'Ornon, OENO, France.
UMR 1366, Bordeaux Sciences Agro, Bordeaux INP, INRAE, ISVV, 33170, Gradignan, OENO, France.
J Physiol Biochem. 2024 Aug;80(3):685-696. doi: 10.1007/s13105-024-01035-w. Epub 2024 Jul 31.
Trans-ε-viniferin, a resveratrol dimer found mainly in grapevine wood, has shown protective capacities against hepatic steatosis in vivo. Nevertheless, this compound is very poorly bioavailable. Thus, the aim of the present study is to determine the potential anti-steatotic properties of 1 and 10 µM of trans-ε-viniferin and its four glucuronide metabolites in AML-12 cells treated with palmitic acid as an in vitro model of hepatic steatosis. The effect of the molecules in cell viability and triglyceride accumulation, and the underlying mechanisms of action by Real-Time PCR and Western Blot were analysed, as well as the quantification of trans-ε-viniferin and the identified bioactive metabolite inside cells and their incubation media. Interestingly, we were able to determine the triglyceride-lowering property of one of the glucuronides (trans-ε-viniferin-2-glucuronide), which acts on de novo lipogenesis, fatty acid uptake and triglyceride assembly. The glucuronides of trans-ε-viniferin would therefore be partly responsible for the in vivo observed anti-steatotic properties of the parent compound.
反式-ε-白藜芦醇二聚体主要存在于葡萄藤木中，已显示出对体内肝脂肪变性的保护作用。然而，这种化合物的生物利用度非常差。因此，本研究旨在确定 1 和 10 μM 的反式-ε-白藜芦醇及其四个葡萄糖醛酸代谢物在以棕榈酸处理的 AML-12 细胞中的潜在抗脂肪变性特性，作为肝脂肪变性的体外模型。通过实时 PCR 和 Western Blot 分析了这些分子对细胞活力和甘油三酯积累的影响，以及作用机制，并对细胞内和孵育培养基中反式-ε-白藜芦醇及其鉴定的生物活性代谢物进行了定量分析。有趣的是，我们能够确定一种葡萄糖醛酸代谢物（反式-ε-白藜芦醇-2-葡萄糖醛酸苷）的降低甘油三酯特性，该代谢物作用于从头脂肪生成、脂肪酸摄取和甘油三酯组装。因此，反式-ε-白藜芦醇的葡萄糖醛酸苷部分负责母体化合物在体内观察到的抗脂肪变性特性。
