Mechanical Engineering Department, IIT Gandhinagar, Palaj, Gandhinagar 382355, Gujarat, India.
Department of Mechanical Engineering, University of Birmingham, Birmingham B15 2TT, U.K.
Langmuir. 2024 Aug 13;40(32):16791-16803. doi: 10.1021/acs.langmuir.4c01173. Epub 2024 Jul 31.
Orthopedic and dental implants made from Ti6Al4V are widely used due to their excellent mechanical properties and biocompatibility. However, the long-term performance of these implants can be compromised by bacterial infections. This study explores the development of hierarchically textured surfaces with enhanced bactericidal properties to address such challenges. Hierarchical surface structures were developed by combining microscale features produced by a microsecond laser and superimposed submicron features produced using a femtosecond laser. Microscale patterns were produced by the pulsed laser surface melting process, whereas submicrometer laser-induced periodic surface structures were created on top of them by femtosecond laser processing. bacterial cells were cultured on the textured surface. After 24 h, a staining analysis was performed using SYTO9 and PI dyes to investigate the samples with a confocal microscope for live dead assays. Results showed bacterial colony formation onto the microscale surface textures with live bacterial cells, whereas the hierarchical surface textures display segregated and physically damaged bacterial cell attachments on surfaces. The hierarchical surface textures showed ∼98% dead bacterial cells due to the combined effect of its multiscale surface features and oxide formation during the laser processing steps. The efficacy of hierarchical surface textures in enhancing the antibacterial behavior of Ti6Al4V implants is evident from the conducted research. Such laser-based surface treatments can find potential applications in different industrial sectors.
钛合金（Ti6Al4V）制成的骨科和牙科植入物由于其优异的机械性能和生物相容性而被广泛应用。然而，这些植入物的长期性能可能会受到细菌感染的影响。本研究旨在探索开发具有增强杀菌性能的分层纹理表面，以解决这些挑战。通过结合微秒激光产生的微尺度特征和飞秒激光产生的亚微米特征来开发分层表面结构。微尺度图案通过脉冲激光表面熔化工艺产生，而亚微米级的激光诱导周期性表面结构则通过飞秒激光加工在其顶部形成。将细菌细胞培养在纹理化表面上。24 小时后，使用 SYTO9 和 PI 染料进行染色分析，并用共聚焦显微镜对活死试验进行研究。结果表明，在微尺度表面纹理上形成了细菌菌落，而在分层表面纹理上，细菌细胞的附着则呈现出分离和物理损伤。由于多尺度表面特征和激光处理过程中形成氧化物的综合作用，分层表面纹理显示出约 98%的死亡细菌细胞。研究表明，分层表面纹理可显著增强 Ti6Al4V 植入物的抗菌性能。这种基于激光的表面处理在不同工业领域可能具有潜在的应用。
