Joolayi Fatemeh, Navidifar Tahereh, Mohammad Jaafari Razieh, Amin Mansour
Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Reproductive Health Promotion Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Int J Reprod Biomed. 2017 Nov;15(11):713-718.
() is the main cause of bacterial sexually transmitted infections. In women, this infection can lead to tubal infertility.
In this study we investigated among infertile and fertile women with both polymerase chain reaction (PCR) and ELISA methods in Ahvaz, Iran.
This case-control study was conducted at the Infertility Clinic of University Jahad, Ahvaz, Iran from January to August 2017. A total of 225 vaginal swabs and blood samples (100 infertile and 125 fertile women) were collected. Detection of DNA was performed from vaginal swabs by amplification of MOMP gene. Also, anti immunoglobulin M (IgM) and immunoglobulin G antibodies in the serum samples were recognized by enzyme-linked immunosorbent assay (ELISA).
Results showed that, 6 (6%) infertile and 2 (1.6%) fertile women were positive for IgM (p=0.21). Also, PCR was positive for infection in 5 infertile (5%) and 2 fertile women (1.6%) (p=0.35). We did not find any seropositive immunoglobulin G in both groups.
In this study, no significant difference was found between fertile and infertile groups for infection. Also, the correlation between IgM and PCR results revealed a relatively strong agreement and seems both PCR and IgM assays are appropriate for the accurate diagnosis of infections.
（）是细菌性性传播感染的主要原因。在女性中，这种感染可导致输卵管性不孕。
在本研究中，我们在伊朗阿瓦士采用聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）方法对不孕和有生育能力的女性进行了调查。
本病例对照研究于2017年1月至8月在伊朗阿瓦士贾哈德大学不孕不育诊所进行。共采集了225份阴道拭子和血液样本（100名不孕女性和125名有生育能力的女性）。通过扩增主要外膜蛋白（MOMP）基因，从阴道拭子中检测（）DNA。此外，血清样本中的抗（）免疫球蛋白M（IgM）和免疫球蛋白G抗体通过酶联免疫吸附测定（ELISA）进行识别。
结果显示，6名（6%）不孕女性和2名（1.6%）有生育能力的女性IgM呈阳性（p = 0.21）。同样，5名不孕女性（5%）和2名有生育能力的女性（1.6%）的PCR检测（）感染呈阳性（p = 0.35）。两组中均未发现免疫球蛋白G血清阳性。
在本研究中，不孕组和有生育能力组在（）感染方面未发现显著差异。此外，IgM与PCR结果之间的相关性显示出相对较强的一致性，似乎PCR和IgM检测均适用于准确诊断（）感染。
