Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Mikrochim Acta. 2024 Aug 1;191(8):498. doi: 10.1007/s00604-024-06588-7.
A novel 3D magnetic nanocomposite material based on covalent organic polymers was successfully synthesized and utilized as an efficient sorbent for magnetic solid-phase extraction. It exhibited a regular core-shell structure, large specific surface area, superior stability, and paramagnetism. To evaluate its extraction efficiency, six flavonoids were tested, demonstrating maximum adsorption capacities ranging from 90 to 218 mg/g. Additionally, the material exhibited remarkable reusability and mechanical stability, maintaining its original state over eight cycles with consistent recovery. An analytical strategy combining magnetic solid-phase extraction with high performance liquid chromatography and tandem mass spectrometry was developed for the determination of flavonoids in orange, honey, soybean, and Dioscorea bulbifera L. samples. The low limits of detection (0.01-0.1 ng/mL) and limits of quantification (0.05-0.5 ng/mL), as well as satisfactory recovery (80.4-114.8%), were obtained. The linear range started from the limits of quantification to 500 ng/mL with R ≥ 0.9929. These results suggest that the prepared adsorbent possesses excellent adsorption capabilities for flavonoids, highlighting its significant potential for detecting these compounds in complex sample matrices.
一种基于共价有机聚合物的新型 3D 磁性纳米复合材料被成功合成，并用作高效的磁性固相萃取吸附剂。它具有规则的核壳结构、大的比表面积、优越的稳定性和顺磁性。为了评估其萃取效率，测试了六种黄酮类化合物，其最大吸附容量范围为 90-218mg/g。此外，该材料表现出显著的可重复使用性和机械稳定性，在八次循环中保持其原始状态，回收率一致。结合磁性固相萃取和高效液相色谱串联质谱，开发了一种用于测定橙、蜂蜜、大豆和黄精样品中黄酮类化合物的分析策略。检测限（0.01-0.1ng/mL）和定量限（0.05-0.5ng/mL）低，回收率（80.4-114.8%）令人满意。线性范围从定量限开始到 500ng/mL，R≥0.9929。这些结果表明，所制备的吸附剂对黄酮类化合物具有优异的吸附能力，突出了其在复杂样品基质中检测这些化合物的巨大潜力。
