State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China.
ACS Nano. 2024 Aug 13;18(32):21077-21090. doi: 10.1021/acsnano.4c02316. Epub 2024 Aug 1.
has been demonstrated to have the strongest association with periodontitis. Within the host, relies on acquiring iron and heme through the aggregation and lysis of erythrocytes, which are important factors in the growth and virulence of . Additionally, the excess obtained heme is deposited on the surface of , protecting the cells from oxidative damage. Based on these biological properties of the interaction between and erythrocytes, this study developed an erythrocyte membrane nanovesicle loaded with gallium porphyrins to mimic erythrocytes. The nanovesicle can target and adhere with precisely, being lysed and utilized by as erythrocytes. Ingested gallium porphyrin replaces iron porphyrin in , causing intracellular metabolic disruption. Deposited porphyrin generates a large amount of reactive oxygen species (ROS) under blue light, causing oxidative damage, and its lethality is enhanced by bacterial metabolic disruption, synergistically killing . Our results demonstrate that this strategy can target and inhibit , reduce its invasion of epithelial cells, and alleviate the progression of periodontitis.
已被证明与牙周炎有最强的关联。在宿主中， 依赖于通过聚集和裂解红细胞来获取铁和血红素，这是 生长和毒力的重要因素。此外，获得的多余血红素沉积在 的表面，保护细胞免受氧化损伤。基于 与红细胞相互作用的这些生物学特性，本研究开发了一种载有镓卟啉的红细胞膜纳米囊泡来模拟红细胞。纳米囊泡可以与 精确地靶向和黏附，被 作为红细胞裂解和利用。摄入的镓卟啉取代 中的铁卟啉，导致细胞内代谢紊乱。沉积的卟啉在蓝光下产生大量的活性氧（ROS），造成氧化损伤，其致死率通过细菌代谢紊乱增强，协同杀死 。我们的结果表明，这种策略可以靶向和抑制 ，减少其对上皮细胞的侵袭，并缓解牙周炎的进展。
