Zhao Luqing, Deng Xin, Ding Ning, Liu Yanzhen, Ma Yun, Li Jiake, Wang Shuqing, Ji Xingyu, Wang Jinjuan, Wang Dianpeng, Li Yanhui, Dou Dan, Zhu Xihan, Zhu Lixin, Zhang Shengsheng
Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China.
Beijing University of Chinese Medicine, Beijing 100105, China.
iScience. 2024 Jun 28;27(8):110403. doi: 10.1016/j.isci.2024.110403. eCollection 2024 Aug 16.
Constipation and frailty are associated with intestinal dysbiosis. This study aims to identify intestinal microbial signatures that can differentiate between constipated elders accompanied by frailty and those without frailty. We collected stool samples from 61 participants and conducted 16S rRNA gene sequencing. Constipated patients with frailty (Constipation_F) exhibited reduced gut microbial diversities compared to constipated patients without frailty (Constipation_NF) and healthy individuals (C). From differential genera, random forest models identified 14, 8, and 5 biomarkers for distinguishing Constipation_F from Constipation_NF, Constipation_F from C, and Constipation_NF from C, respectively. Functional analysis revealed that pathways (P381-PWY and PWY-5507) related to vitamin B12 synthesis were reduced in Constipation_F, which aligns with the decreased abundances of vitamin-B12-producing and in this group. Our study unveils substantial differences in gut microbiota between constipated elders with frailty and those without, underscoring the diagnostic and therapeutic potential of genera involved in vitamin B12 synthesis.
便秘和虚弱与肠道菌群失调有关。本研究旨在确定能够区分伴有虚弱的便秘老年人和不伴有虚弱的便秘老年人的肠道微生物特征。我们收集了61名参与者的粪便样本，并进行了16S rRNA基因测序。与不伴有虚弱的便秘患者（便秘_NF）和健康个体（C）相比，伴有虚弱的便秘患者（便秘_F）的肠道微生物多样性降低。通过差异菌属，随机森林模型分别确定了14种、8种和5种生物标志物，用于区分便秘_F与便秘_NF、便秘_F与C以及便秘_NF与C。功能分析表明，与维生素B12合成相关的途径（P381-PWY和PWY-5507）在便秘_F中减少，这与该组中产生维生素B12的[菌属名称未给出]丰度降低一致。我们的研究揭示了伴有虚弱的便秘老年人和不伴有虚弱的便秘老年人之间肠道微生物群的显著差异，强调了参与维生素B12合成的菌属在诊断和治疗方面的潜力。
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