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对产后健康母马中的马疱疹病毒 1(EHV-1)进行分子检测。

Molecular testing for equine herpesviruses 1 (EHV-1) in healthy postpartum broodmares.

机构信息

Departments of Clinical Studies (Arroyo, Gomez) and Pathobiology (Papapetrou, Lillie), Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1; Ontario Ministry of Agriculture, Food and Rural Affairs, 5th Floor NW, 1 Stone Road West, Guelph, Ontario N1G 4Y2 (Moore).

出版信息

Can Vet J. 2024 Aug;65(8):813-816.

Abstract

OBJECTIVE

Our objective was to determine whether equine herpesviruses 1 (EHV-1) viral nucleic acids could be detected immediately after foaling from nasal and vaginal swabs, whole blood, and placental tissue of healthy mares.

ANIMALS PROCEDURE AND RESULTS

Nasal and vaginal swabs, EDTA blood, and placental tissue (296 samples) were collected from 74 clinically healthy postpartum broodmares within 24 h after giving birth to live, clinically healthy foals. All samples were tested (PCR) for nucleic acids of neuropathogenic and non-neuropathogenic strains of EHV-1, and all were negative.

CONCLUSION AND CLINICAL RELEVANCE

As EHV-1 was not detected in the immediate postpartum period in healthy mares with uncomplicated foaling, we inferred that EHV-1-positive samples from aborting mares and/or EHV-1 detection in fetal membranes indicate EHV-1-associated abortion.

摘要

目的

我们的目的是确定在健康母马分娩后立即从鼻腔和阴道拭子、全血和胎盘组织中是否可以检测到马疱疹病毒 1(EHV-1)病毒核酸。

动物程序和结果

在分娩后 24 小时内,从 74 头临床健康的产后母马中采集了鼻腔和阴道拭子、EDTA 血液和胎盘组织(296 个样本)。所有样本均进行了神经致病性和非神经致病性 EHV-1 毒株的核酸检测(PCR),均为阴性。

结论和临床相关性

由于在没有复杂分娩的健康母马的产后即刻期未检测到 EHV-1,我们推断来自流产母马的 EHV-1 阳性样本和/或在胎膜中检测到 EHV-1 表明与 EHV-1 相关的流产。

相似文献

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Detection of equine herpesviruses in aborted foetuses by consensus PCR.通过共识PCR检测流产胎儿中的马疱疹病毒。
Vet Microbiol. 2008 Jan 1;126(1-3):20-9. doi: 10.1016/j.vetmic.2007.06.019. Epub 2007 Jun 28.

本文引用的文献

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EHV-1: A Constant Threat to the Horse Industry.马疱疹病毒1型:对养马业的持续威胁。
Front Microbiol. 2019 Dec 3;10:2668. doi: 10.3389/fmicb.2019.02668. eCollection 2019.
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Equine herpesvirus-1 consensus statement.马疱疹病毒1型共识声明。
J Vet Intern Med. 2009 May-Jun;23(3):450-61. doi: 10.1111/j.1939-1676.2009.0304.x.

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