National Centre for Veterinary Type Cultures, ICAR-National Research Centre On Equines, Hisar, India.
Department of Veterinary Microbiology, Rajasthan University of Veterinary and Animal Sciences, Navaina, Udaipur, India.
Trop Anim Health Prod. 2024 Aug 2;56(7):226. doi: 10.1007/s11250-024-04089-6.
Since 2019, Lumpy skin disease (LSD) has suddenly spread in many Asian countries, including India. LSD primarily occurs in cattle. However, recent LSD outbreaks in India have also revealed significant morbidity and production losses in buffaloes. This has raised concerns about the role of buffaloes in the epidemiology and transmission of LSD and necessitates the inclusion of buffaloes in the mass vaccination program for the prevention and control of the disease in the country. However, there is no significant data on the immune response in buffaloes following vaccination with the LSD vaccine. In this study, we evaluated antibody- and cell-mediated immune responses following vaccination with a newly developed live-attenuated LSD vaccine (Lumpi-ProVac). The detectable amount of anti-LSDV antibodies was observed at 1-2 months following vaccination, with a peak antibody titer at 3 months. Upon stimulation of the peripheral blood mononuclear cells (PBMCs) with the UV-inactivated LSDV antigen, there was a significant increase in CD8 + T cell counts in vaccinated animals as compared to the unvaccinated animals. Besides, vaccinated animals also showed a significant increase in IFN-γ levels upon antigenic stimulation of their PBMCs with LSDV antigen. In conclusion, the buffaloes also mount a potent antibody- and cell-mediated immune response following vaccination with Lumpi-ProVac.
自 2019 年以来，块状皮肤病（LSD）突然在包括印度在内的许多亚洲国家传播。LSD 主要发生在牛身上。然而，印度最近 LSD 的爆发也表明水牛的发病率和生产损失显著。这引起了人们对水牛在 LSD 的流行病学和传播中的作用的关注，并需要将水牛纳入该国 LSD 疾病预防和控制的大规模疫苗接种计划中。然而，关于接种 LSD 疫苗后水牛的免疫反应的重要数据仍然缺乏。在这项研究中，我们评估了一种新开发的减毒活 LSD 疫苗（Lumpi-ProVac）接种后的抗体和细胞介导的免疫反应。接种后 1-2 个月即可检测到抗 LSDV 抗体的数量，3 个月时抗体滴度达到峰值。在用紫外线灭活的 LSDV 抗原刺激外周血单核细胞（PBMC）后，与未接种疫苗的动物相比，接种疫苗的动物的 CD8+T 细胞计数显著增加。此外，用 LSDV 抗原刺激 PBMC 后，接种疫苗的动物的 IFN-γ 水平也显著增加。总之，水牛接种 Lumpi-ProVac 后也会产生强烈的抗体和细胞介导的免疫反应。
