National Centre for Veterinary Type Cultures, ICAR-National Research Centre on Equines, Hisar, India.
Indian Veterinary Research Institute, Mukteswar, India.
Virulence. 2023 Dec;14(1):2190647. doi: 10.1080/21505594.2023.2190647.
Lumpy skin disease (LSD) was reported for the first time in India in 2019 and since then, it has become endemic. Since a homologous (LSD-virus based) vaccine was not available in the country, goatpox virus (GPV)-based heterologous vaccine was authorized for mass immunization to induce protection against LSD in cattle. This study describes the evaluation of safety, immunogenicity and efficacy of a new live-attenuated LSD vaccine developed by using an Indian field strain, isolated in 2019 from cattle. The virus was attenuated by continuous passage ( = 50) in Vero cells. The vaccine (50 LSDV passage in Vero cells, named as Lumpi-ProVac) did not induce any local or systemic reaction upon its experimental inoculation in calves ( = 10). At day 30 post-vaccination (pv), the vaccinated animals were shown to develop antibody- and cell-mediated immune responses and exhibited complete protection upon virulent LSDV challenge. A minimum Neethling response (0.018% animals; 5 out of 26,940 animals) of the vaccine was observed in the field trials conducted in 26,940 animals. There was no significant reduction in the milk yield in lactating animals ( = 10108), besides there was no abortion or any other reproductive disorder in the pregnant animals ( = 2889). Sero-conversion was observed in 85.18% animals in the field by day 30 pv.
2019 年，印度首次报告了块状皮肤病（LSD），此后该病已成为地方性疾病。由于该国没有同源（基于 LSD 病毒）疫苗，因此授权使用山羊痘病毒（GPV）基于异源疫苗对牛进行大规模免疫接种，以诱导对 LSD 的保护。本研究描述了一种新的减毒 LSD 疫苗的安全性、免疫原性和效力评估，该疫苗是使用 2019 年从牛中分离的印度田间分离株通过连续传代（ = 50）在 Vero 细胞中开发的。该疫苗（在 Vero 细胞中连续传代 50 代的 LSDV，命名为 Lumpi-ProVac）在小牛（ = 10）的实验接种中未引起任何局部或全身反应。在接种疫苗后 30 天（pv），接种疫苗的动物表现出抗体和细胞介导的免疫反应，并在接种强毒 LSDV 后完全受到保护。在对 26940 只动物进行的现场试验中，观察到疫苗的最小 Neethling 反应（0.018%的动物；26940 只动物中有 5 只）。在哺乳期动物（ = 10108）中，产奶量没有明显减少，在妊娠动物（ = 2889）中也没有流产或任何其他生殖障碍。在 pv 第 30 天，通过现场观察到 85.18%的动物发生血清转化率。
