Postgraduate Program in Virology, Evandro Chagas Institute, Secretariat of Health and Environmental Surveillance, Ananindeua, Pará, Brazil.
Virology Section, Evandro Chagas Institute, Secretariat for Health Surveillance and Environment, Ananindeua, Pará, Brazil.
PLoS One. 2024 Aug 2;19(8):e0296568. doi: 10.1371/journal.pone.0296568. eCollection 2024.
Acute gastroenteritis (AGE) is a common pediatric infection that remains a significant cause of childhood morbidity and mortality worldwide, especially in low-income regions. Thus, the objective of this study was to detect human adenovirus (HAdV) and non-polio enterovirus (NPEV) in fecal samples from the Gastroenteritis Surveillance Network, and to identify circulating strains by nucleotide sequencing. A total of 801 fecal samples were tested using qPCR/RT-qPCR, and 657 (82.0%) were inoculated into HEp-2C and RD cell lines. The HAdV and NPEV positivity rates obtained using qPCR/RT-qPCR were 31.7% (254/801) and 10.5% (84/801), respectively, with 5.4% (43/801) co-detection. Cytopathic effect was observed in 9.6% (63/657) of patients, 2.7% (18/657) associated with HAdV, and 6.2% (41/657) associated with NPEV after testing by ICC-PCR. A comparison of the two methodologies demonstrated an agreement of 93.5% for EVNP and 64.4% for HAdV. These two viruses were detected throughout the study period, with HAdV positivity rates ranging from 41% in Amapá to 18% in Pará. The NEPV varied from 18% in Pará/Rondônia to 3% in Acre. The most affected age group was over 60 months for both HAdV and NPEV. Samples previously positive for rotavirus and norovirus, which did not show a major difference in the presence or absence of diarrhea, fever, and vomiting, were excluded from the clinical analyses of these two viruses. These viruses circulated over five years, with a few months of absence, mainly during the months corresponding to the waves of SARS-CoV-2 infection in Brazil. Five HAdV species were identified (A, B, C, D, and F), with a greater predominance of HAdV-F41 (56.5%) followed by HAdV-C (15.2%). Three NPEV species (A, B, and C) were detected, with serotypes E14 (19.3%) and CVA-24 (16.1%) being the most prevalent. The present study revealed a high diversity of NPEV and HAdV types circulating in children with AGE symptoms in the northern region of Brazil.
急性胃肠炎（AGE）是一种常见的儿科感染，仍然是全球儿童发病率和死亡率的重要原因，特别是在低收入地区。因此，本研究的目的是检测肠道病毒监测网络中粪便样本中的人腺病毒（HAdV）和非脊髓灰质炎肠道病毒（NPEV），并通过核苷酸测序鉴定循环株。共检测了 801 份粪便样本，采用 qPCR/RT-qPCR 检测，657 份（82.0%）接种至 HEp-2C 和 RD 细胞系。qPCR/RT-qPCR 检测 HAdV 和 NPEV 的阳性率分别为 31.7%（254/801）和 10.5%（84/801），5.4%（43/801）为混合感染。9.6%（63/657）的患者出现细胞病变效应，2.7%（18/657）与 HAdV 相关，6.2%（41/657）与 NPEV 相关，经 ICC-PCR 检测。两种方法的比较显示，EVNP 的一致性为 93.5%，HAdV 的一致性为 64.4%。这两种病毒在整个研究期间均有检测到，HAdV 的阳性率从阿马帕州的 41%到帕拉州的 18%不等。NEPV 从帕拉州/朗多尼亚州的 18%到阿克里州的 3%不等。HAdV 和 NPEV 受影响最严重的年龄组均为 60 个月以上。轮状病毒和诺如病毒的检测结果之前为阳性，但在腹泻、发热和呕吐的存在或不存在方面没有明显差异，因此排除了这两种病毒的临床分析。这两种病毒在五年内传播，有几个月的时间没有传播，主要是在巴西 SARS-CoV-2 感染浪潮期间。共鉴定出 5 种 HAdV （A、B、C、D 和 F），其中 HAdV-F41 （56.5%）更为常见，其次是 HAdV-C （15.2%）。检测到 3 种 NPEV （A、B 和 C），其中血清型 E14 （19.3%）和 CVA-24 （16.1%）最为常见。本研究显示，巴西北部地区有症状的 AGE 儿童中存在高度多样化的 NPEV 和 HAdV 类型。
