Computational and Systems Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Computational and Systems Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Trends Biotechnol. 2024 Nov;42(11):1379-1395. doi: 10.1016/j.tibtech.2024.07.006. Epub 2024 Aug 1.
Single cell sequencing technologies have become a fixture in the molecular profiling of cells due to their ease, flexibility, and commercial availability. In particular, partitioning individual cells inside oil droplets via microfluidic reactions enables transcriptomic or multi-omic measurements for thousands of cells in parallel. Complementing the multitude of biological discoveries from genomics analyses, the past decade has brought new capabilities from assay baselines to enable a deeper understanding of the complex data from single cell multi-omics. Here, we highlight four innovations that have improved the reliability and understanding of droplet microfluidic assays. We emphasize new developments that further orient principles of technology development and guidelines for the design, benchmarking, and implementation of new droplet-based methodologies.
单细胞测序技术由于其简便性、灵活性和商业可用性，已成为细胞分子谱分析的主要手段。特别是，通过微流控反应将单个细胞分隔在油滴内，可实现数千个细胞的转录组或多组学平行测量。在基因组学分析带来众多生物学发现的同时，过去十年也从检测基准线带来了新的能力，使人们能够更深入地理解单细胞多组学的复杂数据。在这里，我们重点介绍了四项创新，这些创新提高了液滴微流控分析的可靠性和可理解性。我们强调了新的发展，这些发展进一步确定了技术开发的原则，并为基于液滴的新方法的设计、基准测试和实施提供了指导。
