Arnold G, Liscum L, Holtzman E
J Histochem Cytochem. 1979 Mar;27(3):735-45. doi: 10.1177/27.3.39097.
A recently developed procedure for the localization of D-amino acid oxidase (D-AAO) has been used to investigate the distribution of this enzyme in rat nervous tissue. Initial studies were carried out on kidney to validate the methods. The cytochemically demonstrable enzyme in kidney is inhibited by kojic acid, a known competitive D-AAO inhibitor. Omission of the catalse inhibitor, aminotriazole, from the cytochemical medium produces a marked diminution of D-AAO reaction product in kidney peroxisomes. This would be expected if catalase and D-AAO are present in the same particles. In brain, kojic acid-inhibitable D-AAO is demonstrable in numerous bodies within astrocytes especially in the cerebellum, a brain region known from biochemistry to contain particularly high levels of the oxidase. In preparations incubated for catalase, far fewer positive bodies are seen in the cerebellum. Moreover, omission of aminotriazole has little evident effect on the D-AAO reaction. Thus, the oxidase-containing cerebellar bodies may be relatively poor in catalse. In contrast, several nervous system cell types that contain relatively numerous catalase-positive bodies, contain none with detectable D-AAO. Such heterogeneity of peroxisome enzyme content is in accord with reports from biochemical studies of brain.
一种最近开发的用于定位D-氨基酸氧化酶（D-AAO）的方法已被用于研究该酶在大鼠神经组织中的分布。最初的研究在肾脏上进行以验证该方法。肾脏中细胞化学可显示的酶受到曲酸（一种已知的竞争性D-AAO抑制剂）的抑制。从细胞化学培养基中省略过氧化氢酶抑制剂氨基三唑会导致肾脏过氧化物酶体中D-AAO反应产物显著减少。如果过氧化氢酶和D-AAO存在于同一颗粒中，这是可以预期的。在大脑中，曲酸可抑制的D-AAO在星形胶质细胞内的许多小体中可被显示，尤其是在小脑，从生物化学角度已知该脑区含有特别高水平的氧化酶。在用于检测过氧化氢酶的制剂中，在小脑中看到的阳性小体要少得多。此外，省略氨基三唑对D-AAO反应几乎没有明显影响。因此，含有氧化酶的小脑小体可能过氧化氢酶含量相对较低。相比之下，几种含有相对大量过氧化氢酶阳性小体的神经系统细胞类型，没有检测到含有D-AAO的小体。过氧化物酶体酶含量的这种异质性与大脑生化研究的报告一致。
