Sulyok Michael, Suman Michele, Krska Rudolf
Institute of Bioanalytics and Agro-Metabolomics, Department of Agrobiotechnology, IFA-Tulln, University of Natural Resources and Life Sciences, Vienna, Konrad-Lorenz-Str. 20, 3430, Tulln, Austria.
FFoQSI GmbH, Technopark 1D, 3430, Tulln, Austria.
NPJ Sci Food. 2024 Aug 3;8(1):49. doi: 10.1038/s41538-024-00294-7.
This work reports on the validation of a liquid chromatography-tandem mass spectrometric method for the simultaneous quantification of more than 700 mycotoxins and other secondary fungal metabolites and plant toxins in pasta, biscuits, crackers and musli. The "dilute and shoot" approach was found to be fully applicable to these complex matrices, as only 7-14% of the analytes exhibited significant matrix effects while recoveries of the extraction were outside the target range of 70-120% for only 26 compounds. Data on repeatability (based on 7 brands per matrix) and on intermediate precision was compliant to the related < 20% criterion for 95-98% and 99% of all analytes, respectively. The limits of quantification were much lower than the related regulatory limits set for mycotoxins in cereal products. Application of the method to 157 samples from the European market revealed the presence of enniatins and deoxynivalenol in the majority of the samples. No regulatory limits were exceeded except the sum of ergot alkaloids being higher in a few samples than the 50-150 µg/kg to be implemented as of July 2024.
本研究报告了一种液相色谱 - 串联质谱法的验证情况，该方法用于同时定量分析意大利面、饼干、薄脆饼干和穆兹利（一种谷物棒）中的700多种霉菌毒素、其他真菌次生代谢产物和植物毒素。结果发现，“稀释进样”方法完全适用于这些复杂基质，因为只有7% - 14%的分析物表现出显著的基质效应，而只有26种化合物的萃取回收率超出了70% - 120%的目标范围。重复性数据（基于每种基质7个品牌）和中间精密度数据分别符合所有分析物中95% - 98%和99%的相关<20%标准。定量限远低于谷物产品中霉菌毒素的相关监管限量。将该方法应用于157份欧洲市场样品，结果显示大多数样品中存在恩镰孢菌素和脱氧雪腐镰刀菌烯醇。除少数样品中麦角生物碱的总和高于将于2024年7月实施的50 - 150μg/kg限量外，未超过任何监管限量。
