……叶片中活性成分的质谱成像分析

Mass Spectrometry-Imaging Analysis of Active Ingredients in the Leaves of .

作者信息

Zhan Xiaori, Zang Yue, Ma Ruoyun, Lin Wanting, Li Xiao-Lin, Pei Yanyan, Shen Chenjia, Jiang Yan

机构信息

College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.

Zhejiang Provincial Key Laboratory for Genetic Improvement and Quality Control of Medicinal Plants, Hangzhou Normal University, Hangzhou 311121, China.

出版信息

ACS Omega. 2024 Apr 10;9(16):18634-18642. doi: 10.1021/acsomega.4c01440. eCollection 2024 Apr 23.

DOI:10.1021/acsomega.4c01440

PMID:38680336

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11044248/

Abstract

BACKGROUND

is an endangered evergreen conifer mainly found in Northeast Asia. In addition to the well-known taxanes, several active ingredients were detected in the leaves of . However, the precise spatial distribution of active ingredients in the leaves of is largely unknown.

RESULTS

in the present study, timsTOF flex MALDI-2 analysis was used to uncover the accumulation pattern of active ingredients in leaves. In total, 3084 ion features were obtained, of which 944 were annotated according to the mass spectrometry database. The principal component analysis separated all of the detected metabolites into four typical leaf tissues: mesophyll cells, upper epidermis, lower epidermis, and vascular bundle cells. Imaging analysis identified several leaf tissues that specifically accumulated active ingredients, providing theoretical support for studying the regulation mechanism of compound biosynthesis. Furthermore, the relative accumulation levels of each identified compound were analyzed. Two flavonoid compounds, ligustroflavone and Morin, were identified with high content through quantitative analysis of the ion intensity.

CONCLUSIONS

our data provides fundamental information for the protective utilization of .

摘要

背景

[某种植物名称]是一种濒危的常绿针叶树，主要分布在东北亚地区。除了众所周知的紫杉烷类化合物外，在[该植物名称]的叶子中还检测到了几种活性成分。然而，[该植物名称]叶子中活性成分的精确空间分布在很大程度上尚不清楚。

结果

在本研究中，使用timsTOF flex MALDI-2分析来揭示[该植物名称]叶子中活性成分的积累模式。总共获得了3084个离子特征，其中944个根据质谱数据库进行了注释。主成分分析将所有检测到的代谢物分为四种典型的叶组织：叶肉细胞、上表皮、下表皮和维管束细胞。成像分析确定了几个特异性积累活性成分的叶组织，为研究化合物生物合成的调控机制提供了理论支持。此外，还分析了每种鉴定出的化合物的相对积累水平。通过对离子强度的定量分析，鉴定出两种黄酮类化合物——女贞黄酮和桑色素，其含量较高。