Clinical Microbiology, Department of Translational Medicine, Faculty of Medicine, Lund University, Malmö, Sweden; Department of Clinical Microbiology, Infection Control and Prevention, Region Skåne, Lund, Sweden.
Department of Clinical Microbiology, Infection Control and Prevention, Region Skåne, Lund, Sweden; Division of Infection Medicine, Department of Clinical Sciences, Lund University, Lund, Sweden.
J Hosp Infect. 2024 Oct;152:93-98. doi: 10.1016/j.jhin.2024.06.022. Epub 2024 Aug 5.
Irradiation with violet-blue light (VBL), in the spectrum of 405-450 nm, has been reported to be effective against pathogenic bacteria.
To investigate whether VBL irradiation could reduce the level of surface contamination at seven shared patient bathrooms in two wards at a hospital in Sweden.
Repeated sampling of five separate surfaces (door handle, tap water handle, floor, toilet seat, and toilet armrest) was performed in the bathrooms where 405 nm light-emitting diode spotlights had been installed. A prospective study with a cross-over design was carried out, which included two study periods, first with the spotlights either switched on or off and a second study period with the opposite spotlight status.
In total, 665 surface samples were collected during the study (133 samples per surface). Bacterial growth was found in 84% of all samples. The most common findings were coagulase-negative staphylococci and Bacillus spp. The median number of colony-forming units (cfu)/cm was 15 (interquartile range: 5-40) for all surfaces. In our main outcome, mean cfu/cm of all surfaces in a bathroom, no difference was observed with or without VBL. Clean surfaces (<5 cfu/cm) were more commonly observed in bathrooms with VBL, also when controlling for confounding factors. No difference was observed in the number of heavily contaminated surfaces.
This study did not safely demonstrate an additive effect on bacterial surface levels when adding VBL to routine cleaning in shared patient bathrooms.
据报道，波长在 405-450nm 的蓝紫光照射对致病菌有效。
研究蓝紫光照射是否能降低瑞典两家医院两个病房 7 间共用患者浴室中表面污染程度。
在安装了 405nm 发光二极管聚光灯的浴室中，对五个不同表面（门把手、自来水把手、地板、马桶座和马桶扶手）进行了重复采样。采用交叉设计的前瞻性研究，包括两个研究期，聚光灯要么开着，要么关着，然后是相反的聚光灯状态的第二期研究。
在研究期间共采集了 665 个表面样本（每个表面 133 个样本）。所有样本中 84%均发现有细菌生长。最常见的发现是凝固酶阴性葡萄球菌和芽孢杆菌属。所有表面的菌落形成单位（cfu）/cm 的中位数为 15（四分位距：5-40）。在我们的主要结果中，浴室中所有表面的平均 cfu/cm 没有差异，无论是否使用蓝紫光。在有蓝紫光的浴室中，清洁表面（<5cfu/cm）更常见，即使控制了混杂因素也是如此。受污染严重的表面数量没有差异。
本研究未安全证明在共用患者浴室的常规清洁中添加蓝紫光对细菌表面水平有附加作用。
