Chongqing Key Laboratory for New Chemical Materials of Shale Gas, College of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing, 408100, China.
Department of Chemistry, Institute for Molecular Science and Fusion Technology, Kangwon National University, Chuncheon, 24341, Korea.
Anal Bioanal Chem. 2024 Oct;416(24):5295-5302. doi: 10.1007/s00216-024-05460-0. Epub 2024 Aug 5.
Currently, research in the development of high-performance sensing platforms has increased to fulfill the needs of analysis and detection. In this study, we developed a novel type of surface-enhanced Raman scattering (SERS) chip composed of a covalent organic framework (COF)-silver nanoparticles (AgNPs) nanocomposite, and this nanocomposite was fabricated by a one-step method of ultrasonically mixing the obtained COF and AgNPs. The fabricated chip exhibited high sensitivity and repeatable SERS effects. Practical application results showed that the chip was highly sensitive and reliable and capable of detecting DNA bases (adenine) to fit an equation in the range from 0.01 pM to 1 nM, with an R-square of 0.97253 and a detection limit of ~0.026 pM (signal-to-noise ratio (S/N) = 3). Therefore, the proposed SERS system has potential applications in biological assays.
目前，为满足分析和检测的需求，高性能传感平台的研发工作有所增加。在本研究中，我们开发了一种新型的基于共价有机框架（COF）-银纳米粒子（AgNPs）纳米复合材料的表面增强拉曼散射（SERS）芯片，该纳米复合材料是通过超声混合获得的 COF 和 AgNPs 的一步法制备的。所制备的芯片表现出高灵敏度和可重复的 SERS 效应。实际应用结果表明，该芯片具有高灵敏度和可靠性，能够检测 DNA 碱基（腺嘌呤），拟合范围在 0.01 pM 至 1 nM 之间，R-square 为 0.97253，检测限约为 0.026 pM（信噪比（S/N）= 3）。因此，所提出的 SERS 系统在生物分析中具有潜在的应用。
