Lu Zongyan, Guo Hao, Wang Mingyue, Yang Zeyun, Wei Xiaoqin, Liu Yinsheng, Hui Yingfei, Yu Zhiguo, Yang Wu
Key Laboratory of Eco-Functional Polymer Materials of the Ministry of Education, Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Gansu International Scientific and Technological Cooperation Base of Water-Retention Chemical Functional Materials, Lanzhou, 730070, People's Republic of China.
Anal Bioanal Chem. 2023 Nov;415(27):6785-6798. doi: 10.1007/s00216-023-04955-6. Epub 2023 Sep 21.
The development of a simple and sensitive electrochemical sensing platform for levofloxacin (LVF) analysis is of great significance to human health. In this work, a covalent organic framework (TP-COF) was in situ grown on the surface of Sn-MoC nanospheres with nanoflower-like morphology through a one-pot method to obtain the TP-COF@Sn-MoC composite. The prepared composite was used to modify a glassy carbon electrode (GCE) to realize the sensitive detection of levofloxacin. TP-COF was formed by polycondensation of 2,4,6-tris(4-aminophenyl)-1,3,5-triazine (TAPT) and pyromellitic dianhydride (PMDA), in which C = O and C = N groups served as double active centers for the recognition and electrocatalytic oxidation of the target molecule. Meanwhile, the introduction of Sn-MoC improved the conductivity of the electrode. The TP-COF@Sn-MoC composite produced a strong synergistic effect and showed a high electrocatalytic ability toward levofloxacin oxidation. The linear range of LVF was 0.6-1000 μM and the limit of detection (LOD) was 0.029 μM (S/N = 3). In addition, the sensor has been successfully applied for the analysis of LVF in human urine and blood serum samples with acceptable recovery rates, demonstrating that the sensor was promising in practical applications.
开发一种用于左氧氟沙星（LVF）分析的简单灵敏的电化学传感平台对人类健康具有重要意义。在这项工作中，通过一锅法在具有纳米花状形态的Sn-MoC纳米球表面原位生长共价有机框架（TP-COF），以获得TP-COF@Sn-MoC复合材料。将制备的复合材料用于修饰玻碳电极（GCE），以实现对左氧氟沙星的灵敏检测。TP-COF由2,4,6-三（4-氨基苯基）-1,3,5-三嗪（TAPT）和均苯四甲酸二酐（PMDA）缩聚而成，其中C=O和C=N基团作为目标分子识别和电催化氧化的双活性中心。同时，Sn-MoC的引入提高了电极的导电性。TP-COF@Sn-MoC复合材料产生了很强的协同效应，对左氧氟沙星氧化表现出高电催化能力。LVF的线性范围为0.6-1000μM，检测限（LOD）为0.029μM（S/N=3）。此外，该传感器已成功应用于人尿和血清样品中LVF的分析，回收率良好，表明该传感器在实际应用中具有前景。
