Yamada H, Sano Y
Arch Histol Jpn. 1985 Oct;48(4):343-54. doi: 10.1679/aohc.48.343.
The distribution of serotonin neuronal somata in the brain of the rabbit was demonstrated by use of immunoperoxidase technique. The serotonin antibody obtained from a rabbit was applied with the two-step ABC (avidin-biotin-peroxidase complex) method after biotinylation of this antibody. The location, shape, size and immunoreactivity of these somata were clarified in our permanent preparations without non-specific and background staining. These perikarya were distributed in the raphe nuclei, linear nuclei and interpeduncular nucleus, within and around the medial lemniscus, in the mesencephalic periaqueductal grey lateral to the raphe dorsalis, and in the reticular formation of the brain stem. However the so-called "masked indolamine cells" were not observed in the hypothalamus or the area postrema, as our experimental animals were not given any pharmacological pretreatment. These results were essentially similar to those from other mammalian species, especially the rat.
采用免疫过氧化物酶技术显示了家兔脑中5-羟色胺神经元胞体的分布。用从家兔获得的5-羟色胺抗体，经生物素化后采用两步ABC（抗生物素蛋白-生物素-过氧化物酶复合物）法。在我们的永久制片中，这些胞体的位置、形态、大小和免疫反应性得以明确，且无非特异性和背景染色。这些核周体分布在中缝核、线性核和脚间核，在内侧丘系内及周围，在中缝背侧外侧的中脑导水管周围灰质，以及脑干的网状结构中。然而，由于我们的实验动物未进行任何药理学预处理，在下丘脑或最后区未观察到所谓的“隐匿性吲哚胺细胞”。这些结果与其他哺乳动物物种尤其是大鼠的结果基本相似。
