二环己基碳二亚胺和丁二酸钠会引起与龙眼果肉分解相关的能量代谢变化。

Dicyclohexylcarbodiimide and disodium succinate induce the change of energy metabolism in relation to longan pulp breakdown.

作者信息

Zheng Yi, Lin Hetong, Sang Yueying, Lin Mengshi, Lin Yixiong, Wang Hui, Fan Zhongqi, Chen Yihui, Lin Yifen

机构信息

Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.

Key Laboratory of Postharvest Biology of Subtropical Special Agricultural Products, Fujian Province University, Fuzhou, Fujian 350002, China.

出版信息

Food Chem X. 2024 Jul 2;23:101609. doi: 10.1016/j.fochx.2024.101609. eCollection 2024 Oct 30.

Compared to control longan, DCC-treated longan had higher pulp breakdown index, lower ATP, ADP and EC levels, and lower H, Ca and Mg-ATPase activities. On day 6, DCC-treated longan presented 18% higher pulp breakdown index, with 44%, 9% and 31% lower levels of ATP, ADP and EC, respectively. Additionally, DCC-treated longan showed 29%, 53%, 37% lower activity of H-ATPase, 34%, 54%, 4% lower activity of Ca-ATPase, and 13%, 21%, 6% lower activity of Mg-ATPase in the membranes of plasma, vacuole, and mitochondria, respectively. Whereas, DS-treated longan manifested the opposite trends of DCC treatment. These results suggest that the accelerated pulp breakdown in DCC-treated longan was linked to energy deficiency and reduced energy production. However, DS treatment restrained pulp breakdown occurrence in fresh longan by maintaining a higher energy level through the elevated energy production and ATPase activity.

与对照龙眼相比，经二环己基碳二亚胺（DCC）处理的龙眼具有更高的果肉软化指数、更低的三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和电导率（EC）水平，以及更低的氢离子（H）、钙离子（Ca）和镁离子（Mg）-ATP酶活性。在第6天，经DCC处理的龙眼果肉软化指数高出18%，而ATP、ADP和EC水平分别降低了44%、9%和31%。此外，经DCC处理的龙眼在质膜、液泡膜和线粒体膜中的H-ATP酶活性分别降低了29%、53%、37%，Ca-ATP酶活性分别降低了34%、54%、4%，Mg-ATP酶活性分别降低了13%、21%、6%。然而，经壳聚糖（DS）处理的龙眼表现出与DCC处理相反的趋势。这些结果表明，经DCC处理的龙眼果肉加速软化与能量缺乏和能量产生减少有关。然而，DS处理通过提高能量产生和ATP酶活性来维持较高的能量水平，从而抑制了新鲜龙眼果肉软化的发生。