Maroteaux L, Herzog M, Soyer-Gobillard M O
Biosystems. 1985;18(3-4):307-19. doi: 10.1016/0303-2647(85)90031-0.
The 5.8 S rRNA gene of Prorocentrum micans, a primitive dinoflagellate, has been cloned and its 159 base pairs (bp) have been sequenced along with the two flanking internal transcribed spacers (ITS 1 and 2), respectively, 212 and 195 bp long. Nucleotide sequence homologies between several previously published 5.8 S rRNA gene sequences including those from another dinoflagellate, an ascomycetous yeast, protozoans, a higher plant and a mammal have been determined by sequence alignment. Two prokaryotic 5'-ends of the 23 S rRNA gene have been compared owing to their probable common origin with eucaryotic 5.8 S rRNA genes. Several nucleotides are distinctive for dinoflagellates when compared with either typical eucaryotes or procaryotes. This is consistent with an early divergence of the dinoflagellate lineage from the typical eucaryotes. The secondary structure of dinoflagellate 5.8 S rRNA molecules fits the model of Walker et al. (1983). Conserved nucleotides which distinguish dinoflagellate 5.8 S rRNA from that of other eucaryotes are located in specific loops which are assumed to play a structural role in the ribosome. A 5.8 S rRNA phylogenetic tree which is proposed, based on sequence data, supports our initial assumption of the dinoflagellates.
已克隆了原始双鞭毛藻米氏原甲藻（Prorocentrum micans）的5.8 S核糖体RNA基因，并对其159个碱基对（bp）以及两个侧翼的内部转录间隔区（ITS 1和ITS 2）进行了测序，ITS 1和ITS 2分别长212 bp和195 bp。通过序列比对确定了几个先前发表的5.8 S核糖体RNA基因序列之间的核苷酸序列同源性，这些序列包括来自另一种双鞭毛藻、一种子囊酵母、原生动物、一种高等植物和一种哺乳动物的序列。由于23 S核糖体RNA基因的两个原核生物5′端可能与真核生物5.8 S核糖体RNA基因有共同起源，因此对其进行了比较。与典型的真核生物或原核生物相比，双鞭毛藻的几个核苷酸具有独特性。这与双鞭毛藻谱系从典型真核生物中早期分化一致。双鞭毛藻5.8 S核糖体RNA分子的二级结构符合沃克等人（1983年）的模型。将双鞭毛藻5.8 S核糖体RNA与其他真核生物的5.8 S核糖体RNA区分开来的保守核苷酸位于特定的环中，这些环被认为在核糖体中起结构作用。基于序列数据提出的一个5.8 S核糖体RNA系统发育树支持了我们对双鞭毛藻的初步假设。
