Department of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
J Bioenerg Biomembr. 2024 Oct;56(5):505-515. doi: 10.1007/s10863-024-10033-y. Epub 2024 Aug 5.
This study investigated Cerium oxide nanoparticles (CeONPs) effect on central neuropathic pain (CNP). The compressive method of spinal cord injury (SCI) model was used for pain induction. Three groups were formed by a random allocation of 24 rats. In the treatment group, CeONPs were injected above and below the lesion site immediately after inducing SCI. pain symptoms were evaluated using acetone, Radian Heat, and Von Frey tests weekly for six weeks. Finally, we counted fibroblasts using H&E staining. We evaluated the expression of Cx43, GAD65 and HDAC2 proteins using the western blot method. The analysis of results was done by PRISM software. At the end of the study, we found that CeONPs reduced pain symptoms to levels similar to those observed in normal animals. CeONPs also increased the expression of GAD65 and Cx43 proteins but did not affect HDAC2 inhibition. CeONPs probably have a pain-relieving effect on chronic pain by potentially preserving GAD65 and Cx43 protein expression and hindering fibroblast infiltration.
本研究探讨了氧化铈纳米粒子（CeONPs）对中枢神经性疼痛（CNP）的影响。采用脊髓损伤（SCI）模型的压缩方法诱导疼痛。通过随机分配，将 24 只大鼠分为三组。在治疗组中，在诱导 SCI 后立即在损伤部位上方和下方注射 CeONPs。每周使用丙酮、辐射热和 Von Frey 试验评估疼痛症状 6 周。最后，我们使用 H&E 染色计算成纤维细胞数量。我们使用 Western blot 方法评估 Cx43、GAD65 和 HDAC2 蛋白的表达。结果分析使用 PRISM 软件进行。研究结束时，我们发现 CeONPs 可将疼痛症状减轻至与正常动物相似的水平。CeONPs 还增加了 GAD65 和 Cx43 蛋白的表达，但不影响 HDAC2 的抑制。CeONPs 可能通过潜在地维持 GAD65 和 Cx43 蛋白的表达和阻止成纤维细胞浸润来发挥对慢性疼痛的缓解作用。
