Department of basic sciences, Saveh University of Medical Sciences, Saveh, Iran.
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Science, Kerman, Iran.
J Mater Sci Mater Med. 2023 Feb 21;34(2):9. doi: 10.1007/s10856-023-06711-9.
Since the CNS is unable to repair itself via neuronal regeneration in adult mammals, alternative therapies need to be found. The use of cerium oxide nanoparticles to repair nerve damage could be a promising approach for spinal cord reconstruction. In this study, we constructed a scaffold containing cerium oxide nanoparticles (Scaffold-CeO) and investigated the rate of nerve cell regeneration in a rat model of spinal cord injury. The scaffold of gelatin and polycaprolactone was synthesized, and a gelatin solution containing cerium oxide nanoparticles was attached to the scaffold. For the animal study, 40 male Wistar rats were randomly divided into 4 groups (n = 10): (a) Control; (b) Spinal cord injury (SCI); (c) Scaffold (SCI + scaffold without CeO nanoparticles); (d) Scaffold-CeO (SCI + scaffold containing CeO nanoparticles). After creation of a hemisection SCI, scaffolds were placed at the site of injury in groups c and d, and after 7 weeks the rats were subjected to behavioral tests and then sacrificed for preparation of the spinal cord tissue to measure the expression of G-CSF, Tau and Mag proteins by Western blotting and Iba-1 protein by immunohistochemistry. The result of behavioral tests confirmed motor improvement and pain reduction in the Scaffold-CeO group compared to the SCI group. Decreased expression of Iba-1 and higher expression of Tau and Mag in the Scaffold-CeO group compared to the SCI group could be the result of nerve regeneration caused by the scaffold containing CeONPs as well as relief of pain symptoms.
由于中枢神经系统（CNS）在成年哺乳动物中无法通过神经元再生来自我修复，因此需要寻找替代疗法。使用氧化铈纳米颗粒修复神经损伤可能是脊髓重建的一种有前途的方法。在这项研究中，我们构建了一种含有氧化铈纳米颗粒的支架（支架-CeO），并研究了它在大鼠脊髓损伤模型中促进神经细胞再生的速度。合成了明胶和聚己内酯的支架，并将含有氧化铈纳米颗粒的明胶溶液附着在支架上。在动物研究中，将 40 只雄性 Wistar 大鼠随机分为 4 组（n = 10）：（a）对照组；（b）脊髓损伤（SCI）组；（c）支架组（SCI + 不含 CeO 纳米颗粒的支架）；（d）支架-CeO 组（SCI + 含有 CeO 纳米颗粒的支架）。在创建半横断 SCI 后，将支架放置在 c 组和 d 组的损伤部位，7 周后，对大鼠进行行为测试，然后处死以制备脊髓组织，通过 Western blot 法测量 G-CSF、Tau 和 Mag 蛋白的表达，通过免疫组织化学法测量 Iba-1 蛋白的表达。行为测试的结果证实，与 SCI 组相比，支架-CeO 组的运动功能改善和疼痛减轻。与 SCI 组相比，支架-CeO 组的 Iba-1 表达减少，Tau 和 Mag 表达增加，这可能是由于含有 CeONPs 的支架促进了神经再生以及缓解了疼痛症状。
