Shanghai Med-X Engineering Center for Medical Equipment and Technology School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030, P. R. China.
ACS Nano. 2024 Aug 20;18(33):22080-22094. doi: 10.1021/acsnano.4c05333. Epub 2024 Aug 5.
Meningeal vascular network is significant in neurology and neurosurgery. However, high-resolution imaging of intact meningeal vascular network is lacking. In this work, we develop a practical experimental method to ensure that the intact meninges are morphologically unfolded and fixed in an agarose gel. With the help of high-brightness polymer dots (Pdots) as probe, macroscopic and detailed imaging of the vascular network on the intact dorsal meninges can be performed. Meningeal vessels are symmetrically distributed along the superior sagittal sinus, and the distribution of meningeal vessels had a certain degree of hierarchy. The meninges are thicker blood vessels and capillary networks from the outside to the inside. Moreover, the diameter of the capillaries is 3.96 ± 0.89 μm. Interestingly, meningeal primo vessels in the central nervous system of mice is imaged with the diameter of 4.18 ± 1.18 μm, which has not been reported previously. It is worth mentioning that we found that orthotopic xenografts of brain tumors caused the appearance of corneal neovascularization and morphological changes in optic nerve microvessels. In conclusion, our work provides an effective Pdots-based imaging method for follow-up research on meningeal vascular-related diseases, and illustrates that the eye can serve as a window for the prevention and diagnosis of brain diseases.
脑膜血管网络在神经科学和神经外科学中具有重要意义。然而，完整脑膜血管网络的高分辨率成像仍然缺乏。在本工作中，我们开发了一种实用的实验方法，以确保完整脑膜在琼脂糖凝胶中形态展开和固定。借助高亮度聚合物点（Pdots）作为探针，可以对完整脑膜背侧的血管网络进行宏观和详细成像。脑膜血管沿上矢状窦呈对称分布，脑膜血管的分布具有一定的层次结构。脑膜从外到内依次为较厚的血管和毛细血管网络。此外，毛细血管的直径为 3.96±0.89μm。有趣的是，我们成功地对小鼠中枢神经系统中的脑膜原血管进行成像，其直径为 4.18±1.18μm，这是以前未曾报道过的。值得一提的是，我们发现脑肿瘤的原位异种移植导致了角膜新生血管的出现和视神经微血管的形态变化。总之，我们的工作为脑膜血管相关疾病的后续研究提供了一种有效的基于 Pdots 的成像方法，并表明眼睛可以作为预防和诊断脑部疾病的窗口。
