Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Adv Healthc Mater. 2022 Nov;11(21):e2200978. doi: 10.1002/adhm.202200978. Epub 2022 Sep 4.
Ocular diseases are mainly caused by vascular aberrations in the eye, and accurate imaging and analysis of the ocular vascular structure is crucial. In this study, poly(9,9-dioctylfluorene-alt-benzothiadiazole) (PFBT) polymer dots (Pdots), with the advantages of easy synthesis, high brightness, and low toxicity, are used as nanoprobes to perform high-resolution imaging of the vasculature of the eyeball and optic nerve. Moreover, rapid imaging of the choroidal microvessels is carried out by stereoscopic fluorescence microscopy with a resolution of up to 1.6 µm. The comprehensive 3D vascular information of retinal aorta and optic nerve microvessels is obtained by combining tissue clearing and multiphoton microscopy. In addition, the vascular density of Schlemm's canal and iris blood vessels is compared between the conjunctivitis mice and the normal mice. These results suggest that PFBT Pdots have great application potential in the fast and accurate imaging of ocular diseases.
眼部疾病主要是由眼部血管异常引起的，因此准确成像和分析眼部血管结构至关重要。在这项研究中，聚（9,9-二辛基芴--alt-苯并噻二唑）（PFBT）聚合物点（Pdots）具有合成简单、亮度高、毒性低等优点，被用作纳米探针，对眼球和视神经的血管进行高分辨率成像。此外，通过具有 1.6 µm 分辨率的立体荧光显微镜对脉络膜微血管进行快速成像。通过组织透明化和多光子显微镜相结合，获得视网膜主动脉和视神经微血管的综合 3D 血管信息。此外，还比较了结膜炎小鼠和正常小鼠的施莱姆氏管和虹膜血管的血管密度。这些结果表明，PFBT Pdots 在眼部疾病的快速准确成像方面具有很大的应用潜力。
