Band J D, Alvarado C J, Maki D G
Am J Clin Pathol. 1979 Dec;72(6):980-4. doi: 10.1093/ajcp/72.6.980.
Using steel intravneous needles obtained from patients with hematologic malignancies, the authors evaluated the efficacy of a semiquantitative method for culturing vascular cannulas on solid medium, comparing it with conventional broth culture. Of 148 needles studied, 140 (95%) were negative on semiquantitative culture (less than 15 colonies on the plate) although 38 produced growth in broth or on the plate; none of these needles caused septicemia. Eight needles were positive on semiquantitative culture (greater than or equal to 15 colonies), and two of these caused septicemia (P = .002). Positive semiquantitative cultures were associated with local inflammation (P = .02). Semiquantitative culturing was equal to the broth method in sensitivity (100%) in the diagnosis of needle-related septicemia; specificity (96% vs. 92%) and the predictive value of a positive needle culture (25% vs. 14%) were both enhanced with the semiquantitative method. The semiquantitative technic differentiates infection from contamination and offers other major advantages compared with the broth method, and is recommended for culturing steel needles as well as plastic catheters.
作者使用从血液系统恶性肿瘤患者身上获取的钢制静脉针，评估了一种在固体培养基上培养血管插管的半定量方法的有效性，并将其与传统肉汤培养法进行比较。在研究的148根针中，140根（95%）半定量培养呈阴性（平板上菌落少于15个），尽管有38根在肉汤或平板上有生长；这些针均未引起败血症。8根针半定量培养呈阳性（菌落大于或等于15个），其中2根引起了败血症（P = 0.002）。半定量培养阳性与局部炎症相关（P = 0.02）。在诊断与针相关的败血症时，半定量培养在敏感性（100%）方面与肉汤法相当；半定量法提高了特异性（96%对92%）和针培养阳性的预测价值（25%对14%）。半定量技术能够区分感染与污染，与肉汤法相比还有其他主要优势，推荐用于钢制针以及塑料导管的培养。
