Paya C V, Guerra L, Marsh H M, Farnell M B, Washington J, Thompson R L
Division of Infectious Diseases and Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905.
J Clin Microbiol. 1989 Jul;27(7):1431-3. doi: 10.1128/jcm.27.7.1431-1433.1989.
The use of a differential quantitative blood culture technique (Isolator) to diagnose intravascular-device-related bacteremia (IDRB) was studied prospectively. During septic episodes in 44 patients, blood was obtained simultaneously through the suspected infected device and from a peripheral venipuncture. The blood samples were processed by the Isolator technique, which enables easy quantification of microorganisms. The cannula was removed, and its tip was cultured semiquantitatively. Of the 52 cannulas studied, 15 were the cause of IDRB, but only 7 of these showed a significantly higher bacterial count in blood obtained through the device compared with peripheral blood. The bacterial count was higher in blood drawn through the device than in peripheral blood in four of six cases that did not fulfill the definition of IDRB. Some blood cultures obtained through the device were positive despite negative cultures of peripheral blood and cannula tips (six cannulas). Quantitative blood cultures were not useful in diagnosing IDRB in this study.
前瞻性地研究了使用差异定量血培养技术（隔离器）诊断血管内装置相关菌血症（IDRB）的情况。在44例患者发生败血症期间，同时通过疑似感染的装置和外周静脉穿刺采集血液。血样采用隔离器技术处理，该技术能够轻松对微生物进行定量。移除插管，并对其尖端进行半定量培养。在研究的52根插管中，15根是IDRB的病因，但其中只有7根插管所采集血液中的细菌计数明显高于外周血。在6例不符合IDRB定义的病例中，有4例通过装置采集的血液中的细菌计数高于外周血。尽管外周血和插管尖端培养结果为阴性，但通过装置采集的一些血培养结果呈阳性（6根插管）。在本研究中，定量血培养对诊断IDRB并无帮助。
