Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Int J Biol Macromol. 2024 Oct;277(Pt 3):134485. doi: 10.1016/j.ijbiomac.2024.134485. Epub 2024 Aug 3.
The blood glucose concentration in aquatic organisms, a crucial indicator reflecting their health status, holds significant importance for detecting glucose levels in serum in terms of processing and quality monitoring. In this study, a novel POD biomimetic enzyme (p-BEs) with horseradish peroxidase catalytic properties was designed, optimized, and its mechanism was discussed in detail. Based on this, a portable system has been developed capable of determining glucose levels in three ways: quantitatively analyzed through UV-Vis/MD, quantitatively analyzed on-site using a mobile phone RGB, and semi-quantitatively analyzed through a drip plate. Meanwhile, compared with other catalytic methods for detecting glucose, we achieved a lower limit of detection (0.03 μM) and shorter detection time (12 min), with high catalytic activity. This study provides new insights into the design of efficient and reliable cascade catalytic systems responsive to glucose, offering a low-cost, simplicity of operation method for glucose detection.
水生生物的血糖浓度是反映其健康状况的关键指标，对于血清中葡萄糖水平的检测和质量监控具有重要意义。本研究设计、优化了一种具有辣根过氧化物酶催化特性的新型过氧化物酶模拟酶（p-BEs），并详细探讨了其作用机制。在此基础上，开发了一种便携式系统，能够通过三种方式测定葡萄糖水平：通过紫外可见分光光度法/吸光度（MD）进行定量分析，使用手机 RGB 进行现场定量分析，以及通过滴板进行半定量分析。与其他检测葡萄糖的催化方法相比，我们实现了更低的检测限（0.03 μM）和更短的检测时间（12 分钟），具有较高的催化活性。本研究为设计高效、可靠的葡萄糖响应级联催化系统提供了新的思路，为葡萄糖检测提供了一种低成本、操作简便的方法。
