Division of Regenerative Dental Medicine and Periodontology, University Clinics of Dental Medicine, University of Geneva, Geneva, Switzerland.
Periodontology, Faculty of Odontology, Malmo University, Malmo, Sweden.
Clin Exp Dent Res. 2024 Aug;10(4):e946. doi: 10.1002/cre2.946.
The placement of ligatures in the cervical area of rat molars is considered as a predictable model to induce periodontitis.
The present explorative study aimed to compare the efficacy of metal wires (MWs), without or with sandblasting, versus silk ligatures (SLs) in inducing periodontal bone loss in rats.
Twenty-four Wistar rats were randomly divided into three groups of eight rats that received three different types of ligatures (MW, sandblasted wire [SMW], and SL) around their first right mandibular molar, while the contralateral tooth was left without the ligature and served as a control. Bone loss was assessed by measuring the distance from the cementoenamel junction (CEJ) to the bone crest at the distal aspect of the first molar on central mesiodistal sections generated from micro-CT scans taken 24 and 35 days after ligature placement.
In the SL group, only in two rats the ligatures were retained until the end of the 24-day period; in all other animals, the ligatures were lost at some time point. In the SMW, the ligatures were retained only for the 24-day period. In the MW group, no ligatures were lost. Irrespective of the group or experimental period, the difference in the crestal bone level between ligated and control teeth was in most cases z < 0.20 mm, that is, in 19 out of 25 pairs of teeth. In a few cases, the bone crest was more apically located at the control teeth compared to the ligated ones (four cases each, during both 24- and 35-day experimental periods).
Bone loss was minimal during the experimental period, with no significant differences between the test and control teeth, or among the three types of ligatures. MWs, not even roughened, do not seem to be a better alternative to SLs for inducing bone loss in the experimental periodontitis model in the rat. This assumption, however, has to be confirmed in a larger, well-powered study.
在大鼠磨牙颈部放置结扎线被认为是一种可预测的诱导牙周炎的模型。
本探索性研究旨在比较金属丝（MW）、无喷砂金属丝（SMW）和丝线结扎（SL）在诱导大鼠牙周骨丢失方面的效果。
24 只 Wistar 大鼠随机分为三组，每组 8 只大鼠，在第一右下颌磨牙周围分别使用三种不同类型的结扎线（MW、喷砂金属丝[SMW]和 SL），而对侧牙齿不结扎作为对照。通过测量微 CT 扫描获得的中-近中矢状面第一磨牙远中牙槽嵴顶到釉牙骨质界的距离，评估骨丢失情况。微 CT 扫描分别在结扎后 24 天和 35 天进行。
在 SL 组，只有 2 只大鼠的结扎线保留到 24 天；在所有其他动物中，结扎线在某个时间点丢失。在 SMW 组，结扎线仅保留 24 天。在 MW 组，结扎线未丢失。无论组间还是实验期间，结扎牙和对照牙之间的牙槽骨嵴顶水平差异在大多数情况下 z < 0.20mm，即 25 对牙齿中有 19 对。在少数情况下，对照牙的牙槽骨嵴顶比结扎牙更向根尖方向（24 天和 35 天实验期间各有 4 对）。
在实验期间，骨丢失量很小，结扎牙和对照牙之间，或三种结扎线之间无显著差异。即使未经喷砂处理的 MW 似乎也不是诱导大鼠牙周炎模型中骨丢失的 SL 的更好替代品。然而，这一假设需要在更大的、有足够效力的研究中得到证实。
