Zhang Xuyang, Xu Minglu, Xue Qin, He Yao
Department of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing, China.
Chongqing Key Laboratory of Oral Disease and Biomedical Sciences, Stomatological Hospital of Chongqing Medical University, Chongqing, China.
Front Bioeng Biotechnol. 2023 Jan 11;10:1098015. doi: 10.3389/fbioe.2022.1098015. eCollection 2022.
Periodontitis is a prevalent disease caused teeth lost. The present rat models inducing periodontitis with thread ligature and metal steel ligature have some disadvantages. We modified the existing rat ligature periodontitis model by fixing the thread ligature on the metal steel ligature passed through the gap between the first and second molars of rats with detailed modeling steps and illustrations. We research the pathological process of the periodontitis induced by the modified model, and briefly compared the modified model with the thread ligature model and the metal steel ligature model. Our experimental results showed that there was an aggravation in inflammatory infiltration and alveolar bone resorption in modeling area within 14 days of initial induction. After that, the inflammatory infiltration was reduced. And no significant increase in alveolar bone destruction appeared. The modified model was more reliable compared to the thread ligature model, and had greater ability of bacterial aggregation compared to the metal steel ligature model. The modified method covered pathological process of the periodontitis, and showed sufficient efficiency and reliability in inducing rat periodontitis.
牙周炎是一种导致牙齿缺失的常见疾病。目前使用丝线结扎和金属丝结扎诱导大鼠牙周炎的模型存在一些缺点。我们通过将丝线结扎固定在穿过大鼠第一和第二磨牙之间间隙的金属丝上，对现有的大鼠结扎性牙周炎模型进行了改进，并给出了详细的建模步骤和图示。我们研究了改进模型诱导的牙周炎的病理过程，并简要比较了改进模型与丝线结扎模型和金属丝结扎模型。我们的实验结果表明，在初次诱导后的14天内，建模区域的炎症浸润和牙槽骨吸收加剧。之后，炎症浸润减少。并且牙槽骨破坏没有明显增加。与丝线结扎模型相比，改进模型更可靠，与金属丝结扎模型相比，具有更强的细菌聚集能力。改进方法涵盖了牙周炎的病理过程，在诱导大鼠牙周炎方面显示出足够的效率和可靠性。
