Samsun Veterinary Control Institute, Ministry of Agriculture and Forestry, Atakum, 55200, Samsun, Türkiye.
Department of Laboratory Animals, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, 55139, Samsun, Türkiye.
Arch Virol. 2024 Aug 6;169(9):173. doi: 10.1007/s00705-024-06103-6.
In this study, seven bee viruses of significant importance for bee health in Türkiye were investigated using one-step RT-PCR. For this purpose, larvae from 1183 hives and adult bees from 1196 hives were sampled from 400 apiaries in 40 provinces. The prevalence of viral infections in hives was as follows: acute bee paralysis virus (ABPV), 6.4%; black queen cell virus (BQCV), 77%; chronic bee paralysis virus (CBPV), 3.2%; deformed wing virus (DWV), 63.8%; Israel acute bee paralysis virus (IAPV), 7%; Kashmir bee virus (KBV), 2.7%; sacbrood virus (SBV), 49.7%. Moreover, 50 different combinations of viral infections were identified in the hives. While dual infections (36.1%) were the most common in hives, triple infections with BQCV, DWV, and SBV were found to have the highest prevalence (22.1%). At least one viral infection was detected in all of the apiaries tested. Phylogenetic analysis showed that the isolates from this study generally exhibited the highest similarity to previously reported Turkish isolates. When similarity ratios and the locations and types of amino acid mutations were analyzed, it was observed that the isolates from our study exhibited high similarity to isolates from various countries, including China, the United Kingdom, Syria, and Germany.
在这项研究中，使用一步 RT-PCR 方法研究了对土耳其蜜蜂健康具有重要意义的七种蜜蜂病毒。为此，从 40 个省份的 400 个养蜂场中采集了 1183 个蜂群的幼虫和 1196 个蜂群的成年蜜蜂。蜂群中病毒感染的流行率如下：急性麻痹病毒（ABPV），6.4%；黑皇后细胞病毒（BQCV），77%；慢性麻痹病毒（CBPV），3.2%；变形翅膀病毒（DWV），63.8%；以色列急性麻痹病毒（IAPV），7%；克什米尔蜜蜂病毒（KBV），2.7%；囊状幼虫病病毒（SBV），49.7%。此外，在蜂群中还鉴定出 50 种不同的病毒组合感染。虽然双重感染（36.1%）在蜂群中最为常见，但 BQCV、DWV 和 SBV 的三重感染的流行率最高（22.1%）。在所测试的所有养蜂场中均检测到至少一种病毒感染。系统进化分析表明，本研究中的分离株与先前报道的土耳其分离株具有最高的相似性。当分析相似性比率以及氨基酸突变的位置和类型时，发现本研究中的分离株与包括中国、英国、叙利亚和德国在内的多个国家的分离株具有高度相似性。
