Department of Virology, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic.
Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech Republic.
Viruses. 2019 Mar 29;11(4):306. doi: 10.3390/v11040306.
Instrumental insemination of L. queens is a widely employed technique used in honeybee breeding that enables the effective control of mating. However, drone semen represents a potential source of honeybee viruses. In this study, 43 semen doses collected from apparently healthy drones, and consequently used in instrumental insemination, were analysed using PCR or RT-PCR to detect the presence of viral genome of 11 honeybee viruses. In 91% of samples, viral infection was detected. The survey revealed genomes of five viruses, namely Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), Sacbrood virus (SBV), and filamentous virus (FV) in 84%, 19%, 14%, 2%, and 67% of samples, respectively. Single infection (30% of samples) as well as multiple infection (61% of samples) of two, three or four pathogens were also evaluated. To the best of our knowledge, this is the first study describing the presence of the BQCV and SBV genome sequence in drone ejaculate. Phylogenetic analysis of BQCV partial helicase gene sequence revealed the high similarity of nucleotide sequence of described Czech strains, which varied from 91.4% to 99.6%. The findings of our study indicate the possibility of venereal transmission of BQCV and SBV.
仪器授精 L. queens 是一种广泛应用于蜜蜂养殖的技术，可有效控制交配。然而，雄蜂精液是蜜蜂病毒的潜在来源。在这项研究中，分析了 43 份来自明显健康雄蜂的精液样本，这些样本随后被用于仪器授精，使用 PCR 或 RT-PCR 检测 11 种蜜蜂病毒的病毒基因组。在 91%的样本中检测到病毒感染。调查显示，在 84%、19%、14%、2%和 67%的样本中分别检测到了五种病毒的基因组，即变形翅膀病毒（DWV）、急性蜜蜂麻痹病毒（ABPV）、黑蜂王细胞病毒（BQCV）、Sacbrood 病毒（SBV）和丝状病毒（FV）。还评估了单一感染（30%的样本）和两种、三种或四种病原体的多重感染（61%的样本）。据我们所知，这是首次描述 BQCV 和 SBV 基因组序列存在于雄蜂精液中的研究。BQCV 部分解旋酶基因序列的系统发育分析表明，描述的捷克株的核苷酸序列高度相似，差异从 91.4%到 99.6%不等。我们的研究结果表明，BQCV 和 SBV 可能通过性行为传播。
