Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.
Department of Life Science, Aberystwyth University, Aberystwyth, UK.
Sci Rep. 2024 Aug 7;14(1):18278. doi: 10.1038/s41598-024-69373-9.
To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.
为了解析调控种子萌发的分子基础，本研究揭示了帽结合复合物（CBC）在调节大麦中脱落酸（ABA）抑制作用方面的关键作用，CBC 由 CBP20 和 CBP80 组成。通过使用编码 CBC 的基因的单和双大麦突变体，我们发现双突变体 hvcbp20.ab/hvcbp80.b 对 ABA 不敏感，与在萌发过程中观察到的单突变体的超敏性形成鲜明对比。我们的全面转录组和代谢组分析不仅鉴定了基因表达和剪接模式的显著变化，还强调了 CBC、ABA 和油菜素内酯（BR）信号通路之间的调控关系。
