Lin Fanfan, Cai Yifan, Yang Shihai, Yang Yunqiang
Germplasm Bank of Wild Species, Yunnan Key Laboratory for Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Xishuangbanna 666303, China.
Genes (Basel). 2025 Mar 18;16(3):350. doi: 10.3390/genes16030350.
(1) : , a drought-tolerant plant native to the Tibetan Plateau, plays a crucial ecological and economic role. While its drought tolerance mechanisms have been extensively studied, the role of alternative splicing (AS) in drought resistance remains insufficiently explored. This study aims to elucidate how AS events regulate gene expression to enhance drought tolerance in under water-deficit conditions. (2) : plants were subjected to progressive drought stress followed by rehydration. Physiological responses, transcriptomic data, and hormonal profiles were analyzed to investigate the plant's adaptive mechanisms to drought stress, with a particular focus on abscisic acid (ABA) signaling-related genes. (3) : The results showed that maintained high leaf water content even under severe drought stress, emphasizing its strong drought resistance. A transcriptomic analysis revealed 11,962 differentially expressed genes, primarily enriched in hormone signaling and metabolic pathways. Notably, the accumulation of ABA was closely associated with AS events in ABA-related genes, such as , , and . These genes produced multiple splice variants, indicating their role in modulating the ABA signaling pathway and enhancing drought tolerance. (4) : This study highlights the pivotal role of AS in ABA signaling and drought tolerance in . It provides new insights into how AS contributes to plant adaptation to drought stress, bridging the knowledge gap in drought resistance mechanisms and emphasizing the importance of AS in plant stress responses.
(1)：[植物名称]是一种原产于青藏高原的耐旱植物，发挥着关键的生态和经济作用。虽然其耐旱机制已得到广泛研究，但可变剪接（AS）在抗旱中的作用仍未得到充分探索。本研究旨在阐明在水分亏缺条件下，AS事件如何调控基因表达以增强[植物名称]的耐旱性。(2)：对[植物名称]植株进行渐进性干旱胁迫，随后复水。分析生理反应、转录组数据和激素谱，以研究植物对干旱胁迫的适应性机制，特别关注脱落酸（ABA）信号相关基因。(3)：结果表明，即使在严重干旱胁迫下，[植物名称]仍保持较高的叶片含水量，突显其强大的抗旱性。转录组分析揭示了11962个差异表达基因，主要富集于激素信号和代谢途径。值得注意的是，ABA的积累与ABA相关基因（如[基因名称1]、[基因名称2]和[基因名称3]）中的AS事件密切相关。这些基因产生多种剪接变体，表明它们在调节ABA信号通路和增强耐旱性方面的作用。(4)：本研究强调了AS在[植物名称]的ABA信号和耐旱性中的关键作用。它为AS如何促进植物对干旱胁迫的适应提供了新见解，弥合了抗旱机制方面的知识差距，并强调了AS在植物应激反应中的重要性。
