The Effect of Electrospun Scaffold Loaded With Taraxasterol Microspheres on the Proliferation and Differentiation of Osteoblasts.

This study aims to observe the effect of electrospun scaffolds loaded with taraxasterol microspheres on the proliferation and differentiation of osteoblasts. Taraxasterol microspheres were prepared by desolventization and electrostatic adsorption technology. The structure of the microspheres was observed under transmission electron microscopy. The drug microspheres were loaded into electrospinning using electrospinning technology, followed by scanning electron microscopy and elemental analysis. Osteoblasts were cultured in vitro, and the effects of drug carriers on osteoblast proliferation and differentiation were observed through Cell Counting kit 8 (CCK-8) cell proliferation detection and alkaline phosphatase activity detection. Transmission electron microscopy showed that the prepared drug microspheres have a double-layer structure, which can effectively reduce the sudden release of drugs. The electrospinning had a porous three-dimensional structure between them, which was conducive to cell adhesion. After loading microspheres, there was a significant difference in electrospinning diameter and nodular protrusions, energy dispersive spectroscopy (EDS) elemental analysis showed that the proportion of nitrogen elements increased significantly after the addition of microspheres, a CCK-8 detection showed that drug carrier scaffolds loaded with taraxasterol had a promoting effect on osteoblast proliferation (P<0.05). Alkaline phosphatase detection showed that drug carrier scaffolds loaded with taraxasterol can promote early differentiation of osteoblasts (P>0.05). Electrospinning loaded with taraxasterol microspheres can promote osteoblast adhesion, proliferation, and differentiation.