Nagarajan Mahendirakumar, Dayasekaran Vijitha, Jayavel Kavitha, Jayaraj Merlin, Chellaswamy Sreeja, Ranganathan Krishnaraj, Kaliamoorthy Sriram, Chellapandi Sugirtha, Baskaran Radhika, Elumalai Agila
Department of Prosthodontics and Crown and Bridge, Government Dental College and Hospital, Cuddalore, IND.
Department of Periodontics and Implantology, Government Dental College and Hospital, Cuddalore, IND.
Cureus. 2024 Jul 7;16(7):e64039. doi: 10.7759/cureus.64039. eCollection 2024 Jul.
Residual ridge resorption presents obstacles in prosthodontic treatment, affecting denture stability and the success of dental implants. Genetic elements, specifically the single nucleotide polymorphism (SNP) 1772C>T variant within the hypoxia-inducible factor 1 subunit alpha (HIF-1α) gene, are hypothesized to contribute to residual ridge resorption progression. Nevertheless, its impact remains insufficiently investigated, especially within the context of South Indian populations. We sought to investigate the connection between SNP 1772C>T and residual ridge resorption (RRR) among fully edentulous individuals, considering demographic factors, genotyping methodologies, and statistical evaluations.
In a prospective case-control study, we recruited 100 completely edentulous participants from South India. Participants were categorized based on alveolar ridge height. Saliva samples were non-invasively collected for DNA extraction, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was employed to determine genotype distribution using the HphI restriction enzyme. The statistical evaluations comprised the utilization of chi-square and Fisher's exact tests.
We observed no significant variations in genotype distributions between the case and control cohorts (CT: p=0.24; CC: p=0.65; TT: p=0.30). The heterozygous genotype CT was prevalent in both groups.
Although we did not observe significant associations between SNP 1772C>T and RRR, our findings imply a genetic predisposition to residual ridge resorption that warrants further exploration. Variations in genetic susceptibility across ethnicities and the influence of other genetic variants on residual ridge resorption require additional investigation. This study lays the groundwork for personalized prosthodontic care by highlighting the potential of genetic analysis in routine dental practice to improve treatment strategies.
剩余牙槽嵴吸收给口腔修复治疗带来了障碍，影响义齿稳定性和牙种植的成功率。据推测，遗传因素，特别是缺氧诱导因子1α（HIF-1α）基因内的单核苷酸多态性（SNP）1772C>T变异，会导致剩余牙槽嵴吸收的进展。然而，其影响尚未得到充分研究，尤其是在南印度人群的背景下。我们试图研究SNP 1772C>T与全口无牙个体的剩余牙槽嵴吸收（RRR）之间的联系，同时考虑人口统计学因素、基因分型方法和统计评估。
在一项前瞻性病例对照研究中，我们招募了100名来自南印度的全口无牙参与者。参与者根据牙槽嵴高度进行分类。通过非侵入性收集唾液样本进行DNA提取，并采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析，使用HphI限制性内切酶确定基因型分布。统计评估包括使用卡方检验和费舍尔精确检验。
我们观察到病例组和对照组之间的基因型分布没有显著差异（CT：p=0.24；CC：p=0.65；TT：p=0.30）。杂合基因型CT在两组中都很常见。
虽然我们没有观察到SNP 1772C>T与RRR之间存在显著关联，但我们的研究结果表明存在剩余牙槽嵴吸收的遗传易感性，值得进一步探索。不同种族之间遗传易感性的差异以及其他基因变异对剩余牙槽嵴吸收的影响需要进一步研究。本研究通过强调基因分析在常规牙科实践中改善治疗策略的潜力，为个性化口腔修复护理奠定了基础。
