Nguyen Hoang-Ngan, Pertzborn David, Ziadat Rafat, Ernst Günther, Guntinas-Lichius Orlando, Von Eggeling Ferdinand, Hoffmann Franziska
Working Group Innovative Biophotonics, Department of Otorhinolaryngology, Jena University Hospital, D-07747 Jena, Germany.
Biomed Rep. 2024 Jul 26;21(3):136. doi: 10.3892/br.2024.1824. eCollection 2024 Sep.
Indocyanine green (ICG) is a potential promising dye for a better intraoperative tumor border definition and an improved patient outcome by potentially improving tumor border visualization compared with traditional white light guided surgery. Here, the cellular uptake of ICG in human squamous cell carcinoma (SCC026) and immortalized non-cancer skin (HaCaT) cell lines was evaluated to study the tumor-specific cellular uptake of ICG. The spatial distribution of ICG inside tumor tissue was investigated in tissue sections of head and neck squamous cell carcinoma at a microscopic level. ICG uptake and internalization was observed in living cells after 2.5 h and in the nucleus after 24 h. In dead cells, higher and faster uptake was observed. In the tissue sections, higher ICG signal intensity could be detected in connective tissue and surrounding clusters and blood vessels. In conclusion, no distinct ICG uptake by tumor cells was detected in cancer cell lines and tumor tissue. ICG localization in certain regions of tumor tissue appears to be a result of enhanced tissue permeability and retention, but not specific to tumor cells.
吲哚菁绿（ICG）是一种潜在的有前景的染料，与传统白光引导手术相比，它可能通过改善肿瘤边界可视化来更好地在术中界定肿瘤边界并改善患者预后。在此，评估了ICG在人鳞状细胞癌（SCC026）和永生化非癌皮肤（HaCaT）细胞系中的细胞摄取情况，以研究ICG的肿瘤特异性细胞摄取。在显微镜水平上，对头颈部鳞状细胞癌组织切片中肿瘤组织内ICG的空间分布进行了研究。2.5小时后在活细胞中观察到ICG摄取和内化，24小时后在细胞核中观察到。在死细胞中，观察到更高且更快的摄取。在组织切片中，在结缔组织、周围簇和血管中可检测到更高的ICG信号强度。总之，在癌细胞系和肿瘤组织中未检测到肿瘤细胞对ICG的明显摄取。ICG在肿瘤组织某些区域的定位似乎是组织通透性和滞留增强的结果，而非肿瘤细胞特有的。
