伊拉克新冠肺炎患者亚甲基四氢叶酸还原酶（）与5-甲基四氢叶酸-同型半胱氨酸甲基转移酶还原酶（）多态性之间的关联。
Association between Methylenetetrahydrofolate Reductase () and 5-Methyltetrahydrofolate-Homocysteine Methyltransferase Reductase () Polymorphisms in Iraqi Patients with COVID-19.
作者信息
Alsaffar Sura F, Baqer Noor Nihad
机构信息
Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.
Ministry of Science and Technology, Baghdad, Iraq.
出版信息
Iran J Med Sci. 2024 Jul 1;49(7):430-440. doi: 10.30476/IJMS.2023.99555.3162. eCollection 2024 Jul.
BACKGROUND
The methylenetetrahydrofolate reductase () gene is an essential gene in the metabolism of folate-homocysteine. Recently, the level of homocysteine was found to be a significant marker in the follow-up of COVID-19 infection. Thus, this study aimed to detect the effect of genetic polymorphisms for single nucleotide polymorphisms (SNPs) (c.66A>G, c.1298A>C, and c.677CT) on COVID-19 infection.
METHODS
Blood samples were collected from 270 patients with COVID-19 in the medical center of Al-Shifa (Baghdad, Iraq) from November 2020 to March 2021. Tetra-primer amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used for the detection of genotypes of SNPs. The odds ratio (OR) was used to detect the relationship between SNPs and COVID-19 infections. Haplotype analysis was performed by SHEsis software.
RESULTS
There was a significant difference between mild/moderate cases and severe/critical cases for ages (35-45), (46-55), and (56-65) years (P<0.0001, P=0.01, and P=0.006, respectively). The results showed significant differences in the T allele for SNP c.677>C (P<0.0001 and OR=4.58). The C allele for SNP c.1298A>C indicated significant differences (P<0.001 and OR=3.15). Besides, the G allele for SNP c.677C>T showed significant differences (P<0.001 and OR=6.64). Consequently, these SNPs showed a predisposition to the development of COVID-19 infection. With regard to the C-A-A, T-A-A and T-C-G haplotypes indicated significant differences between the control and patient groups. The C-A-A was related to a decreased risk and indicated a protective effect against COVID-19 infection development (P<0.0001 and OR=0.218). The increased risk was associated with T-A-A and T-C-G haplotypes and indicated the risk impact on COVID-19 infection development (P<0.0001, P=0.004, and OR=15.5, OR=6.772, respectively). Furthermore, the linkage disequilibrium (LD) for SNPs was studied, and the complete D' value was 99.
CONCLUSION
The genetic polymorphisms of SNPs (c.66A>G, c.1298A>C, and c.677C>T) in the Iraqi population were associated with COVID-19 infection.
背景
亚甲基四氢叶酸还原酶（）基因是叶酸 - 同型半胱氨酸代谢中的关键基因。近期研究发现，同型半胱氨酸水平是新冠病毒感染随访中的一项重要指标。因此，本研究旨在检测单核苷酸多态性（SNP）（c.66A>G、c.1298A>C和c.677C>T）的基因多态性对新冠病毒感染的影响。
方法
2020年11月至2021年3月期间，在伊拉克巴格达的希法医疗中心收集了270例新冠病毒感染患者的血液样本。采用四引物扩增阻滞突变系统 - 聚合酶链反应（ARMS-PCR）技术检测SNP的基因型。优势比（OR）用于检测SNP与新冠病毒感染之间的关系。通过SHEsis软件进行单倍型分析。
结果
年龄在（35 - 45）岁、（46 - 55）岁和（56 - 65）岁的轻症/中症病例与重症/危重症病例之间存在显著差异（P分别<0.0001、P = 0.01和P = 0.006）。结果显示，SNP c.677>C的T等位基因存在显著差异（P<0.0001，OR = 4.58）。SNP c.1298A>C的C等位基因差异显著（P<0.001，OR = 3.15）。此外，SNP c.677C>T的G等位基因差异显著（P<0.001，OR = 6.64）。因此，这些SNP显示出新冠病毒感染易感性。关于C - A - A、T - A - A和T - C - G单倍型，对照组和患者组之间存在显著差异。C - A - A与风险降低相关，对新冠病毒感染发展具有保护作用（P<0.0001，OR = 0.218）。T - A - A和T - C - G单倍型与风险增加相关，表明对新冠病毒感染发展有风险影响（P分别<0.0001、P = 0.004，OR分别 = 15.5、OR = 6.772）。此外，还研究了SNP的连锁不平衡（LD），完全D'值为99。
结论
伊拉克人群中SNP（c.66A>G、c.1298A>C和c.677C>T）的基因多态性与新冠病毒感染有关。
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