Department of Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Methods Mol Biol. 2024;2845:55-66. doi: 10.1007/978-1-0716-4067-8_5.
Preserving mitochondrial homeostasis is vital, particularly for the energetically demanding and metabolically active nerve cells. Mitophagy, the selective autophagic removal of mitochondria, stands out as a prominent mechanism for efficient mitochondrial turnover, which is crucial for proper neuronal development and function. Dysfunctional mitochondria and disrupted mitophagy pathways have been linked to a diverse array of neurological disorders. The nematode Caenorhabditis elegans, with its well-defined nervous system, serves as an excellent model to unravel the intricate involvement of mitophagy in developing neurons. This chapter describes the use of Rosella biosensor in C. elegans to monitor neuronal mitophagy, providing a user-friendly platform for screening genes and drugs affecting mitophagic pathways under physiological conditions or in the context of neurodevelopmental pathologies.
维持线粒体的稳态至关重要，特别是对于能量需求高且代谢活跃的神经细胞。线粒体自噬，即选择性地自噬清除线粒体，是一种促进线粒体有效周转的重要机制，这对于神经元的正常发育和功能至关重要。功能失调的线粒体和受损的线粒体自噬途径与多种神经紊乱有关。秀丽隐杆线虫作为一种具有明确神经系统的模式生物，非常适合用于揭示线粒体自噬在发育中的神经元中的复杂作用。本章介绍了使用 Rosella 生物传感器在秀丽隐杆线虫中监测神经元的线粒体自噬，为在生理条件下或神经发育病理学背景下筛选影响线粒体自噬途径的基因和药物提供了一个用户友好的平台。
