Herrmann J E, Armstrong A S, Edberg S C
Yale J Biol Med. 1985 Sep-Oct;58(5):421-4.
Current techniques for rapid diagnosis of microbial infections by direct detection of the microbial agent are compared. The techniques include enzyme immunoassay (EIA) tests, immunofluorescence, latex agglutination assays, and nucleic acid hybridization procedures. It is concluded that, for the near future, the preferred methods for rapid diagnosis will be by (1) EIA tests utilizing monoclonal antibodies and improved enzyme detection systems, and (2) improved latex agglutination procedures for certain antigens. Nucleic acid hybridization techniques, as currently performed, will need to be substantially improved to become the methods of choice.
对通过直接检测微生物病原体来快速诊断微生物感染的现有技术进行了比较。这些技术包括酶免疫测定（EIA）试验、免疫荧光、乳胶凝集试验和核酸杂交程序。结论是，在不久的将来，快速诊断的首选方法将是：（1）利用单克隆抗体和改进的酶检测系统的EIA试验，以及（2）针对某些抗原改进的乳胶凝集程序。目前所采用的核酸杂交技术需要大幅改进才能成为首选方法。
