Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain; Institute for Agribiotechnology Research (CIALE), Villamayor, Salamanca, Spain.
Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1, 87 100 Torun, Poland.
Syst Appl Microbiol. 2024 Sep;47(5):126542. doi: 10.1016/j.syapm.2024.126542. Epub 2024 Aug 3.
Several strains were isolated from subsurface soil of the Atacama Desert and were previously assigned to the Micromonospora genus. A polyphasic study was designed to determine the taxonomic affiliation of isolates 4G51, 4G53, and 4G57. All the strains showed chemotaxonomic properties in line with their classification in the genus Micromonospora, including meso-diaminopimelic acid in the cell wall peptidoglycan, MK-9(H) as major respiratory quinone, iso-C and iso-C as major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The 16S rRNA gene sequences of strains 4G51, 4G53, and 4G57 showed the highest similarity (97.9 %) with the type strain of Micromonospora costi CS1-12, forming an independent branch in the phylogenetic gene tree. Their independent position was confirmed with genome phylogenies, being most closely related to the type strain of Micromonospora kangleipakensis. Digital DNA-DNA hybridization and average nucleotide identity analyses between the isolates and their closest phylogenomic neighbours confirmed that they should be assigned to a new species within the genus Micromonospora for which the name Micromonospora sicca sp. nov. (4G51=PCM 3031=LMG 30756) is proposed.
从阿塔卡马沙漠的地下土壤中分离出了几株菌，并先前被归为 Micromonospora 属。设计了一个多相研究来确定菌株 4G51、4G53 和 4G57 的分类归属。所有菌株的化学分类特性都符合它们在 Micromonospora 属中的分类，包括细胞壁肽聚糖中的 meso-二氨基庚二酸、主要呼吸醌 MK-9(H)、主要脂肪酸和二磷酸甘油酯 iso-C 和 iso-C、主要极性脂双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰肌醇。菌株 4G51、4G53 和 4G57 的 16S rRNA 基因序列与 Micromonospora costi CS1-12 的模式株相似度最高（97.9%），在系统发育基因树中形成一个独立的分支。它们的独立地位通过基因组系统发育得到了证实，与 Micromonospora kangleipakensis 的模式株最为密切相关。菌株与最近的系统发育邻居之间的数字 DNA-DNA 杂交和平均核苷酸同一性分析证实，它们应该被分配到 Micromonospora 属中的一个新种，该种被命名为 Micromonospora sicca sp. nov.（4G51=PCM 3031=LMG 30756）。
