Krausse R, Ullmann U
Zentralbl Bakteriol Mikrobiol Hyg A. 1985 Nov;260(3):342-60. doi: 10.1016/s0176-6724(85)80023-7.
A total of 56 strains of Campylobacter jejuni (C. jejuni) isolated from diarrhoeal patients were characterized by biochemical tests. The following reactions were performed: hydrolysis of hippurate, reduction of nitrate and nitrite, activity of deoxyribonuclease, hydrolysis of Tween(R) 40, 60 and 80. Including the hydrolysis of the different Tweens(R), 14 biotypes could be distinguished. 19 out of the 56 strains of C. jejuni and the nomenclatural type strains of C. jejuni, C. coli, C. laridis, C. fetus subsp. fetus, subsp. venerealis, C. faecalis, C. sputorum subsp. sputorum, subsp. mucosalis, subsp. bubulus were examined for the production of volatile (VFA) and non-volatile (NVFA) short-chain fatty acids using high performance liquid chromatography (HPLC) with a column for organic acids (Aminex HPX-87 H). A standard mixture of 21 short-chain fatty acids was taken as reference. By this method 5 biotypes of C. jejuni could be characterized. The most frequent biotype was type 1 (78.9%). All the biotypes produced succinic, acetic and butyric acids. Differences existed in the production of pyruvic, malonic, formic and isobutyric acids. C. jejuni could rapidly and clearly be distinguished from C. coli, C. fetus subspp. and catalase-negative Campylobacter species. No qualitative differences were found between the subspecies of C. fetus, C. sputorum subsp. sputorum and subsp. bubulus, C. sputorum subsp. mucosalis and C. faecalis were characterized by presence of fumaric and malonic acid, respectively.
对从腹泻患者中分离出的56株空肠弯曲菌进行了生化试验鉴定。进行了以下反应：马尿酸盐水解、硝酸盐和亚硝酸盐还原、脱氧核糖核酸酶活性、吐温40、60和80的水解。包括不同吐温的水解在内，可区分出14种生物型。对56株空肠弯曲菌中的19株以及空肠弯曲菌、结肠弯曲菌、拉氏弯曲菌、胎儿弯曲菌胎儿亚种、性病亚种、粪弯曲菌、唾液弯曲菌唾液亚种、粘膜亚种、泡亚种的命名型菌株，使用配备有机酸柱（Aminex HPX - 87 H）的高效液相色谱（HPLC）检测挥发性（VFA）和非挥发性（NVFA）短链脂肪酸的产生。以21种短链脂肪酸的标准混合物作为参照。通过这种方法可鉴定出空肠弯曲菌的5种生物型。最常见的生物型是1型（78.9%）。所有生物型均产生琥珀酸、乙酸和丁酸。丙酮酸、丙二酸、甲酸和异丁酸的产生存在差异。空肠弯曲菌可快速且清晰地与结肠弯曲菌、胎儿弯曲菌亚种区分开来，以及与过氧化氢酶阴性的弯曲菌属物种区分开来。在胎儿弯曲菌的亚种之间未发现定性差异，唾液弯曲菌唾液亚种和泡亚种、唾液弯曲菌粘膜亚种和粪弯曲菌分别以富马酸和丙二酸的存在为特征。
