Singh Geeta, Sundaram Ezhilarasi, Chandra Shaleen, Pandey Rahul, Agrawal Amiya, Tiwari Arunesh Kumar
Department of Oral and Maxillofacial Surgery, Faculty of Dental Science, King George Medical University, Lucknow, Uttar Pradesh 226003 India.
Atal Bihari Vajpayee Medical University, Lucknow, Uttar Pradesh India.
J Maxillofac Oral Surg. 2024 Aug;23(4):831-836. doi: 10.1007/s12663-024-02193-6. Epub 2024 May 10.
Understanding of molecular model of oral carcinogenesis has carried cancer chemotherapy far forward from conventional drug therapies. Small molecule inhibitors have gained acceptance as it has fewer adverse effects and also provide targeted drug therapy. The association of HSP 70 (Heat Shock Protein 70) and BCL 2 (B-cell lymphoma 2) proteins with oral precancer and cancer is already established. However, the complex interaction between these two proteins and how they affect each other's expression is still not understood completely. In our study, we aimed to correlate the expression of HSP 70 and BCL 2 with different histopathological grades of oral precancer and cancer tissue samples using tissue immunohistochemistry.
Tissue samples were taken from a total of 250 patients (100 OPMDs and 150 OSCCs) and subjected to immunohistochemistry using anti-human mouse monoclonal antibodies to HSP70 and BCL2. Immunostaining was done, and the immunostaining intensity distribution (IID) index was calculated.
Immunoreactivity scores for both HSP 70 and BCL 2 correlated with different grades of dysplasia. However, only HSP 70 had a statistically significant association ( = 0.066). We also found that HSP 70 showed an inverse correlation, with higher expression majorly seen in well-differentiated OSCCs.
Our study unveiled the HSP 70-BCL 2 interaction and provides insights about how this might affect drug designing and help overcome therapeutic lags. However, further studies are needed to provide a comprehensive review of such interactions among various small molecules.
对口腔癌发生分子模型的理解使癌症化疗从传统药物治疗向前迈进了一大步。小分子抑制剂因其副作用较少且能提供靶向药物治疗而得到认可。热休克蛋白70（HSP 70）和B细胞淋巴瘤2（BCL 2）蛋白与口腔癌前病变和癌症的关联已经确立。然而，这两种蛋白之间的复杂相互作用以及它们如何相互影响表达仍未完全了解。在我们的研究中，我们旨在通过组织免疫组化将HSP 70和BCL 2的表达与口腔癌前病变和癌组织样本的不同组织病理学分级相关联。
共采集了250例患者的组织样本（100例口腔潜在恶性疾患和150例口腔鳞状细胞癌），并使用抗人小鼠单克隆抗体对HSP70和BCL2进行免疫组化。进行免疫染色，并计算免疫染色强度分布（IID）指数。
HSP 70和BCL 2的免疫反应性评分均与不同程度的发育异常相关。然而，只有HSP 70具有统计学意义的关联（P = 0.066）。我们还发现HSP 70呈负相关，在高分化口腔鳞状细胞癌中主要表现为高表达。
我们的研究揭示了HSP 70 - BCL 2的相互作用，并提供了关于这可能如何影响药物设计以及帮助克服治疗滞后的见解。然而，需要进一步研究以全面综述各种小分子之间的这种相互作用。
