Spino D F
Appl Environ Microbiol. 1985 Nov;50(5):1213-8. doi: 10.1128/aem.50.5.1213-1218.1985.
Only a small percentage of the heterotrophic bacteria encountered in water distribution systems are identifiable, because of these organisms fail to grow on the conventional media used for biochemical characterization. Organisms that would not subculture from the same standard plate count agar used for initial isolation were successfully subcultured on a low-nutrient medium, R3A. These cultures were then inoculated to a modified O/F base medium containing specific substrates. This, combined with a lower incubation temperature (30 degrees C), increased the enzymatic activity of many of the organisms. These reactions established a groundwork for tentative taxonomy.
由于在用于生化特性鉴定的传统培养基上无法生长,在配水系统中遇到的异养细菌只有一小部分能够被识别。那些无法从用于初次分离的同一标准平板计数琼脂上继代培养的微生物,在低营养培养基R3A上成功实现了继代培养。然后将这些培养物接种到含有特定底物的改良O/F基础培养基中。这与较低的培养温度(30摄氏度)相结合,提高了许多微生物的酶活性。这些反应为初步分类学奠定了基础。