针对多重耐药菌的裂解性噬菌体的鉴定：为抗微生物耐药性带来希望之光
Identification of Lytic Phages Against Multidrug-Resistant : Illuminating Hope on Antimicrobial-Resistance.
作者信息
Rahman Sezanur, Hossain Mobarok, Akther Jannat, Mahamud Imtiaz, Khan Tahsin, Haider Arefeen, Islam Shahriar, Mondal Shakhinur Islam, Begum Anowara, Biswas Sudhangshu Kumar, Jubair Mohammad, Rahman Mustafizur
机构信息
Virology Laboratory, Infectious Diseases Division, icddr,b, Dhaka, Bangladesh.
Genome Centre, Infectious Diseases Division, icddr,b, Dhaka, Bangladesh.
出版信息
Phage (New Rochelle). 2024 Jun 21;5(2):91-98. doi: 10.1089/phage.2023.0038. eCollection 2024 Jun.
BACKGROUND
Lytic phages have been considered as a solution to mitigate the emergence of multidrug-resistant bacteria. Nevertheless, finding phages capable of targeting a broad host-range remains a significant challenge.
MATERIALS AND METHODS
Our study introduces two lytic phages isolated from hospital effluent, which are active against extended-spectrum cephalosporin-resistant .
RESULTS
Overnight coculture with host, two purified phage lysates yielded around 3.0 × 10 PFU/mL with an average 0.8 ± 0.2 mm diameter of clear, round, and non-halo plaques in both instances. The genomes of iPHaGe-KPN-11i (177,603 bp, 273 coding sequences [CDS]) and iPHaGe-KPN-12i (178,179 bp, 275 CDS) belong to the genus. Both phages have at least 120 genes with known functions, including 1 endolysin and 2 tRNAs, and are capable of lysing at least 12 distinct bacterial species .
CONCLUSIONS
Most phages are host-specific, whereas our phages can kill multiple bacterial species, enabling their potential use for a broad range of hosts.
背景
裂解性噬菌体被认为是缓解多重耐药菌出现的一种解决方案。然而，找到能够靶向广泛宿主范围的噬菌体仍然是一项重大挑战。
材料与方法
我们的研究介绍了从医院污水中分离出的两种裂解性噬菌体，它们对耐超广谱头孢菌素的细菌具有活性。
结果
与宿主进行过夜共培养后，两种纯化的噬菌体裂解液均产生了约3.0×10 PFU/mL的效价，在两种情况下，平均形成直径为0.8±0.2毫米的清晰、圆形且无晕圈的噬菌斑。iPHaGe-KPN-11i（177,603 bp，273个编码序列[CDS]）和iPHaGe-KPN-12i（178,179 bp，275个CDS）的基因组属于该属。两种噬菌体都至少有120个具有已知功能的基因，包括1种溶菌酶和2个tRNA，并且能够裂解至少12种不同的细菌物种。
结论
大多数噬菌体具有宿主特异性，而我们的噬菌体可以杀死多种细菌物种，使其有可能用于广泛的宿主。
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