Hu Bin, Hu Haibo, Peng Donghui, Wei Zheng, Wang Qiuhong, Kuang Haixue
National Engineering Research Center for Modernization of Traditional Chinese Medicine-Hakka Medical Resources Branch, School of Pharmacy, Gannan Medical University, Ganzhou, China.
Key Laboratory of Chinese Materia Medica, School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China.
Front Nutr. 2024 Jul 25;11:1422857. doi: 10.3389/fnut.2024.1422857. eCollection 2024.
The bulbs of are widely used as food or seasoning (garlic), while they have also been utilized as a famous traditional medicine since ancient eras for the treatment of scabies, tuberculosis, pertussis, diarrhea and dysentery, etc. However, very few studies focus on their abundant aerial parts, which are normally discarded during the harvest season.
The hyperlipidemic mice model has been used to study the lipid-lowering effect of the aerial parts in this article. 180 mice were randomly divided into 18 groups, including blank control (BC), model (Mod), positive control (PC), and low-, medium-, and high-dose groups of the crude extract, petroleum ether, ethyl acetate, n-butanol, and residual water extracts (corresponding to CE, PEE, EAE, NBE, WE), with 10 mice in each group. The preventive effects of the extracts on hyperlipidemic mice lasted for four weeks. Ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) and gas chromatography tandem mass spectrometry (GC-MS/MS) were used to analyze the chemical components of NBE and PEE respectively.
The results of the mice experiment showed that n-butanol extract (NBE) and petroleum ether extract (PEE) from the aerial parts could significantly reduce the contents of total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), alanine transaminase (ALT) and aspartate transaminase (AST) in serum of hyperlipidemic mice, and increase the contents of high density lipoprotein cholesterol (HDL-C). They could enhance the activity of superoxide dismutase (SOD) in liver and reduce the level of malondialdehyde (MDA). At the same time, they could improve steatosis and inflammation of liver cells. The results of phytochemical components analysis showed that NBE was rich in organic acids, flavonoids and nitrogen-containing constituents, while PEE contained organic sulfur compounds, aliphatic acids and derivatives, alkaloids, phytosterols, etc.
These results support that the aerial parts of are an interesting source of bioactive ingredients that may be useful in the prevention and treatment of hyperlipidemia.
[植物名称]的鳞茎被广泛用作食物或调味料（大蒜），自古以来它也作为一种著名的传统药物用于治疗疥疮、肺结核、百日咳、腹泻和痢疾等。然而，很少有研究关注其大量的地上部分，这些地上部分在收获季节通常被丢弃。
本文使用高脂血症小鼠模型来研究地上部分的降血脂作用。180只小鼠随机分为18组，包括空白对照组（BC）、模型组（Mod）、阳性对照组（PC）以及粗提物、石油醚提取物、乙酸乙酯提取物、正丁醇提取物和残留水提取物的低、中、高剂量组（分别对应CE、PEE、EAE、NBE、WE），每组10只小鼠。提取物对高脂血症小鼠的预防作用持续四周。分别采用超高效液相色谱四极杆飞行时间质谱（UPLC-Q/TOF-MS）和气相色谱串联质谱（GC-MS/MS）分析NBE和PEE的化学成分。
小鼠实验结果表明，地上部分的正丁醇提取物（NBE）和石油醚提取物（PEE）可显著降低高脂血症小鼠血清中总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的含量，并增加高密度脂蛋白胆固醇（HDL-C）的含量。它们可增强肝脏中超氧化物歧化酶（SOD）的活性并降低丙二醛（MDA）水平。同时，它们可改善肝细胞的脂肪变性和炎症。植物化学成分分析结果表明，NBE富含有机酸、黄酮类化合物和含氮成分，而PEE含有有机硫化合物、脂肪酸及其衍生物、生物碱、植物甾醇等。
这些结果支持[植物名称]的地上部分是生物活性成分的一个有趣来源，可能对高脂血症的预防和治疗有用。
